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EnzymaticsEnzymatics
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| Product Name | Part No. | Unit Size | Concentration | Notes | ||
| NEW PRODUCTS: | ||||||
| E. coli DNA Ligase | L6090L | 2,500 | 10,000 U/ml | Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG | ||
| T4 RNA Ligase 2 | L6080L | 4,500 | 30,000 U/ml | Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures | ||
| T4 RNA Ligase 2 Truncated | L6070L | 500 | 5,000 U/ml | Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA | ||
| Omni Klentaq® | P7500-HC-L | 8,400 | 42,000 U/ml | Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes | ||
| （High Concentration） | ||||||
| Omni Klentaq® | P7500-LC-L | 8,400 | 4,200 U/ml | Lower concentration – 1 microliter per reaction | ||
| （Low Concentration） | ||||||
| Phoenix Hot Start Taq DNA Polymerase | P7590L | 500 | 5,000 U/ml | Antibody based Hot Start Taq DNA polymerase delivers robust PCR performance with exceptional pre-PCR cycling room temperature stability | ||
| VeraSeq™ 2.0 High-Fidelity DNA Polymerase | P7511L | 500 | 2,000 U/ml | Industry-leading speed, fidelity and robustness delivered via an engineered PCR polymerase | ||
| VeraSeq™ ULtra DNA Polymerase | P7520L | 500 | 2,000 U/ml | High fidelity, speed, and robustness in PCR delivered by an engineered, thermostable DNA polymerase that tolerates uracil templates, nucleotides and primers | ||
| RecA | Y9260L | 1.5 mg | 2.0 mg/ml | Promotes strand exchange of single-stranded DNA fragments with DNA duplex substrates | ||
| Thermolabile Phosphatase | Y9210L | 5,500 | 5,000 U/ml | Removes 5′ and 3′-phosphate groups from DNA | ||
| Thermolabile UDG | G5020L | 500 | 1,000 U/ml | Removes uracil from DNA, leaving AP site | ||
| Poly A Polymerase | P7460L | 1,000 | 5,000 U/ml | Catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA which is useful for making polyA tailed RNA | ||
| Ultra Pure Ligases: | ||||||
| Bitmap E. coli DNA Ligase | L6090L | 2,500 | 10,000 U/ml | Efficiently ligates DNA at nicks and cohesive termini. Blunt-ended termini can be joined in the presence of PEG | ||
| T3 DNA Ligase | L6010L | 900,000 | 3,000,000 U/ml | Works in high ionic strength – to 1.0 M NaCl | ||
| T4 DNA Ligase | L6030-LC-L | 150,000 | 120,000 U/ml | Low concentration overnight ligation format | ||
| T4 DNA Ligase （Rapid） | L6030-HC-L | 240,000 | 600,000 U/ml | “Ultrapure ligase” referenced in Nature Methods （Quail, 1 December 2008） | ||
| Bitmap T4 RNA Ligase 1 | L6050L | 10,000 | 20,000 U/ml | Single-stranded RNA ligase. Also joins single-stranded DNA molecules | ||
| T4 RNA Ligase 2 | L6080L | 4,500 | 30,000 U/ml | Ligates nicks on double stranded DNA and from the 3′ OH of RNA to the 5′ phosphate of DNA in double stranded structures | ||
| Bitmap T4 RNA Ligase 2 Truncated | L6070L | 500 | 5,000 U/ml | Requires a preadenylated 5′ phosphate containing DNA or RNA to ligate to 3′ hydroxyl of RNA | ||
| T7 DNA Ligase | L6020L | 900,000 | 3,000,000 U/ml | 1000 fold higher activity on sticky ends than blunt ends | ||
| Taq DNA Ligase | L6060L | 20,000 | 40,000 U/ml | Thermostable DNA ligase which efficiently seals nicks and discriminates against mismatch ligation | ||
| Ultra Pure Polymerases: | ||||||
| DNA Polymerase I | P7050L | 5,000 | 10,000 U/ml | DNA polymerase with 5′→ 3′ synthesis, | ||
| 5′→ 3′ and 3′→ 5′ exonuclease activities | ||||||
| Klenow （3′→ 5′ exo-） | P7010-HC-L | 10,000 | 50,000 U/ml | Proven choice for DNA labeling, A-tailing | ||
| Klenow （3′→ 5′ exo-） | P7010-LC-L | 10,000 | 5,000 U/ml | Proven choice for DNA labeling, A-tailing | ||
| Klenow Fragment | P7060L | 2,500 | 5,000 U/ml | DNA polymerase useful for end-filling prior to blunt-end ligation | ||
| Mako DNA Polymerase | P7090L | 3,000 | 30,000 U/ml | Exonuclease-deficient polymerase, lacks strand displacement activity | ||
| Manta 1.0 DNA Polymerase | P7140-HC-L | 100,000 | 400,000 U/ml | Thermostable Bacillus DNA Polymerase, strong strand displacement | ||
| （High Concentration） | ||||||
| Manta 1.0 DNA Polymerase | P7140-LC-L | 100,000 | 40,000 U/ml | Thermostable Bacillus DNA Polymerase, strong strand displacement | ||
| （Low Concentration） | ||||||
| Bitmap M MuLV Reverse Transcriptase | P7040L | 100,000 | 200,000 U/ml | Useful polymerase for first-strand DNA synthesis | ||
| Omni Klentaq® | P7500-HC-L | 8,400 | 42,000 U/ml | Engineered Taq Polymerase mutant which enables PCR direct from blood, soil, water and food samples. Increased tolerance to fluorescent intercalating dyes | ||
| Bitmap （High Concentration） | ||||||
| Omni Klentaq® | P7500-LC-L | 8,400 | 4,200 U/ml | Lower concentration – 1 microliter per reaction | ||
| （Low Concentration） | ||||||
| φ29 DNA Polymerase | P7020-HC-L | 2,000 | 100,000 U/ml | Exceptional strand-displacement, fidelity and processivity | ||
| （High Concentration） | ||||||
| φ29 DNA Polymerase | P7020-LC-L | 2,000 | 10,000 U/ml | Exceptional strand-displacement, fidelity and processivity | ||
| Bitmap （Low Concentration） | ||||||
| Phoenix Hot Start Taq DNA Polymerase | P7590L | 500 | 5,000 U/ml | Antibody based Hot Start Taq DNA polymerase delivers robust PCR performance with exceptional pre-PCR cycling room temperature stability | ||
| T4 DNA Polymerase | P7080L | 2,000 | 3,000 U/ml | Proven choice for polishing 5′ and 3′ of ends during DNA cloning | ||
| T7 DNA Polymerase | P7260L | 3,500 | 10,000 U/ml | Highly processive DNA polymerase with exceptionally high rate of synthesis and replication fidelity | ||
| T7 RNA Polymerase | P7180L | 50,000 | 50,000 U/ml | Synthesizes RNA in 5′→ 3′ direction off DNA template | ||
| Taq DSC 2.0 DNA Polymerase | P7110L | 1,250 | 5,000 U/ml | Novel, nucleic acid-based hot start enables high performance PCR | ||
| Taq-B DNA Polymerase | P7250L | 10,000 | 5,000 U/ml | Industry Standard for routine PCR | ||
| Bitmap Bitmap Terminal deoxynucleotidyl Transferase （TdT） | P7070L | 6,000 | 20,000 U/ml | Efficiently extends blunt, 5′-overhanging, single-stranded DNA, and RNA. Useful for 3′ labeling | ||
| VeraSeq™ 2.0 High-Fidelity DNA Polymerase | P7511L | 500 | 2,000 U/ml | Industry-leading speed, fidelity and robustness delivered via an engineered PCR polymerase | ||
| VeraSeq™ ULtra DNA Polymerase | P7520L | 500 | 2,000 U/ml | High fidelity, speed, and robustness in PCR delivered by an engineered, thermostable DNA polymerase that tolerates uracil templates, nucleotides and primers | ||
| DNA Modifying Enzymes: | ||||||
| 10X Uracil Cleavage System | Y9180L | 750 Reactions | N/A | 2 component system which generates single nucleotide gap at location of the uracil residue | ||
| APE 1 | Y9110L | 10,000 | 10,000 U/ml | Cleaves phosphodiester bond in AP site leaving 3′ OH and 5′ phosphate | ||
| E. coli Fpg | Y9070L | 4,000 | 8,000 U/ml | Excises damaged purines from duplex DNA, cleaves AP sites leaving 3′ and 5′ phosphates | ||
| Endonuclease VIII | Y9080L | 10,000 | 10,000 U/ml | Excises damaged pyrimidines from duplex DNA, cleaves AP sites leaving 3′ and 5′ phosphates | ||
| End-Repair Mix | Y9140-HC-L | 75 Reactions | N/A | This formulation is optimized for high DNA concentrations, such as library construction for sequencing | ||
| （High Concentration） | ||||||
| End-Repair Mix | Y9140-LC-L | 200 Reactions | N/A | This formulation is optimized for low DNA concentrations, such as general cloning | ||
| （Low Concentration） | ||||||
| Exonuclease I | X8010L | 30,000 | 20,000 U/ml | Excises bases from single-stranded DNA in 3′→ 5′ direction | ||
| Exonuclease III | X8020L | 50,000 | 100,000 U/ml | Excises bases from duplex DNA in 3′→ 5′ direction | ||
| Bitmap Lambda Exonuclease | X8030L | 10,000 | 5,000 U/ml | Highly processive 5′→ 3′ exonuclease, active on blunt and 5′ recessed ends | ||
| RecA | Y9260L | 1.5 mg | 2.0 mg/ml | Promotes strand exchange of single-stranded DNA fragments with DNA duplex substrates | ||
| T4 Polynucleotide Kinase | Y9040L | 10,000 | 10,000 U/ml | Phosphorylation of 5′ ends of DNA prior to ligation | ||
| Uracil DNA Glycosylase | G5010L | 10,000 | 2,000 U/ml | Enzyme creates abasic sites in Uracil-containing DNA | ||
| Bitmap Thermolabile Phosphatase | Y9210L | 5,500 | 5,000 U/ml | Removes 5′ and 3′-phosphate groups from DNA | ||
| Bitmap Thermolabile UDG | G5020L | 500 | 1,000 U/ml | Removes uracil from DNA, leaving AP site | ||
| Binding Proteins: | ||||||
| E.coli SSB | Y9030L | 1.0 mg | 2.75 mg/ml | Binds single-stranded DNA, useful in primer sequestering, thermostable | ||
| T4 Gene 32 Protein | Y9130L | 1.0 mg | 10 mg/ml | Stabilizes ssDNA regions, increases processivity of some DNA polymerases | ||
Bitmap
| ||||||
| Poly A Polymerase | P7460L | 1,000 | 5,000 U/ml | Catalyzes the addition of AMP from ATP to the 3′ hydroxyl of RNA which is useful for making polyA tailed RNA | ||
| RNAse H | Y9220L | 5,000 | 5,000 U/ml | Cleaves RNA strand of DNA:RNA hybrids. Useful for removing mRNA during second-strand cDNA synthesis | ||
| RNAse Inhibitor | Y9240L | 20,000 | 40,000 U/ml | Porcine-derived non-competitve inhibitor of RNAse A, B, C. Does not inhibit RNAse H activity | ||
| Nucleic Acids: | ||||||
| 10 mM dNTP Solution Mix | N2050-10-L | 100 µmol | 10 mM | |||
| 100 mM dATP Solution | N2010-A-L | 75 µmol | 100 mM | |||
| ATP Solution | N2070-10-L | 50 µmol | 10 mM | |||
| dNTP Solution Mix | N2050L | 100 µmol | 25 mM | |||
| dNTP Solution Set | N2010L | 75 µmol Each | 100 mM |
