產(chǎn)品名稱：BRILLIANT GREEN BILE （2%） BROTH
貨號(hào)：oxoidL CM0031
規(guī)格：500g
產(chǎn)品說(shuō)明：
BRILLIANT GREEN BILE （2%） BROTH
Code: CM0031
Directions
Dissolve 40g in 1 litre of distilled water. Mix well, distribute into containers fitted with Durham’s tubes and sterilise by autoclaving at 121°C for 15 minutes.
Do not autoclave double stregnth medium. The alternative procedure is to heat the dissolved broth at 100°C for 30 minutes1.
Description
This medium was formulated by Durham and Schoenlein2 to selectively recover organisms of the coli-aerogenes group. The bile and brilliant green components inhibit the Gram-positive organisms, whilst the coli-aerogenes group are recognised by the rapid formation of gas during lactose fermentation3. It is important that the inhibitory agents in the medium are balanced with the nutrient and mineral components, so that Clostridium and Bacillus spores do not give false positive reactions in the medium i.e. gas formation. Brilliant Green Bile Broth is used in water, dairy and food analysis4,5,6,7,8.
The addition of 4-methylumbelliferyl-β-D-glucuronide （MUG） （BR0071） to this medium will enhance the detection of Escherichia coli. See MUG Reagent （BR0071） under Biological Reagents for further details.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared tubes of broth at 2-8°C.