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Cy5 Picolyl Azide|Cyanine5 Picolyl Azide

Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concentration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.

In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

structural formula：

Cy5 Picolyl Azide|Cyanine5 Picolyl Azide

Abs/Em Maxima	647/663 nm
Extinction coefficient	250,000
Flow cytometry laser line	633 or 635 nm
Microscopy laser line	633 or 635 nm
Spectrally similar dyes	Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649
Molecular weight	953.15
CAS	N/A
Solubility	Water, DMSO, DMF, MeOH
Purity	>95% (HPLC)
Appearance	Blue solid
Storage conditions	-20C
Shipping conditions	Ambient temperature

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Sulfo-Cyanine7 alkyne
Sulfo-Cyanine5.5 alkyne
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Sulfo-Cyanine3 alkyne
ROX alkyne, 5-isomer
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Cyanine3 alkyne
BDP TMR alkyne
BDP FL alkyne
BDP 630/650 alkyne
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1-Ethynyl pyrene	34993-56-1
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Sulfo-Cyanine5 azide
Sulfo-Cyanine3 azide
ROX azide, 5- isomer
R110 azide, 6- isomer
R110 azide, 5- isomer
Pyrene azide 3
Pyrene azide 2
Pyrene azide 1
Perylene azide
PEP azide
Copper chip + NaHCO3) 2-溴-5-甲基噻吩(含穩(wěn)定劑銅芯和碳酸氫鈉)AmdU (5-azidomethyl-2'-deoxyuridine)
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Sulfo-Cyanine5 tetrazine
Sulfo-Cyanine3 tetrazine
TAMRA amine, 5-isomer
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Sulfo-Cyanine5.5 amine

Sulfo-Cyanine3 amine
FAM amine, 5-isomer
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Cyanine3 amine
BDP TMR amine
BDP FL amine
BDP 630/650 amine