TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefin.
Sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation based labeling.
This reagent possesses good aqueous solubility and stability in biological environments.
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General properties：
Appearance: | dark blue solid |
Molecular weight: | 864.09 |
Molecular formula: | C42H46KN7O7S2 |
Solubility: | good in water, DMF, DMSO |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties：
Excitation maximum, nm: | 646 |
ε, L⋅mol−1⋅cm−1: | 271000 |
Emission maximum, nm: | 662 |
Fluorescence quantum yield: | 0.28 |
CF260: | 0.04 |
CF280: | 0.04 |
產(chǎn)品分類列表
活性染料Reactive dyes |
馬來酰亞胺類染料Dye maleimides | Cy3 maleimide Cy5 maleimide Cy5.5 maleimide Cy7 maleimide Cy7.5 maleimide |
活性染料Catalysts and activators | Ascorbic acid Copper(II)-TBTA complex |
炔烴類染料Dye alkynes | 1-Ethynyl pyrene 3-Ethynyl perylene Cy3 alkyne Cy5 alkyne Cy5.5 alkyne |
酯類活性染料Dye activated esters | Cy3 NHS ester Cy3.5 NHS ester Cy5 NHS ester Cy5.5 NHS ester Cy7 NHS ester Cy7.5 NHS ester Sulfo-Cy3 NHS ester Sulfo-Cy5 NHS ester Sulfo-Cy7 NHS ester 6-ROX NHS ester |
疊氮化合物染料Dye azides | Cy3 azide Cy3.5 azide Cy5 azide Cy5.5 azide Cy7 azide Cy7.5 azide 5-FAM azide, 6-FAM azide PEP azide Perylene azide Pyrene azide 1 Pyrene azide 2 Pyrene azide 3 5-R110 azide 6-R110 azide 5-ROX azide Sulfo-Cy3 azide Sulfo-Cy5 azide 5-TAMRA azide, Coumarin 343 azide |
羧酸類染料Carboxylic acids | Cy3 carboxylic acid Cy5 carboxylic acid Cy5.5 carboxylic acid Cy7 carboxylic acid Cy7.5 carboxylic acid Sulfo-Cy5 carboxylic acid Sulfo-Cy7 carboxylic acid |
氨基類Amino dyes | Cy5 amine Cy7.5 amine |
酰肼類染料Dye hydrazides | Cy5 Boc-hydrazide Cy5 hydrazide |
“點擊”化學(xué)試劑Click Chemistry reagents |
Non-fluorescent alkynes | Pentynoic acid STP ester |
Non-fluorescent azides | Azidobutyric acid NHS ester |
DNA |
亞磷酰胺Phosphoramidites | Alkyne Amidite, hydroxyprolinol Alkyne Phosphoramidite, 5'-terminal DMS(O)MT aminolink C6 Long trebler phosphoramidite Pyrene phosphoramidite dU |
改良三磷酸鹽類Modified triphosphates | Ami1-ddUTP Ami1-dUTP |
其他試劑Miscellaneous reagents |
蛋白質(zhì)組學(xué)試劑Proteomic reagents | 3Dye™ 2D DIGE Kits （注：此染料試劑盒能與Ettan Dige （GE Healthcare）配合使用，或替代使用。） Cy2 NHS ester for 2D electrophoresis Cy3 NHS ester for 2D electrophoresis Cy5 NHS ester for 2D electrophoresis |
輔助試劑Auxiliary reagents | Ascorbic acid Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO |
染料SYBR Green I | SYBR Green I Gel Staining Solution, 10000x SYBR Green I for Real Time PCR, 100x |
