英文名稱：FITC-Streptavidin,Fluorescein-Streptavidin
中 文 名：熒光素標記鏈霉親和素，FITC標記的鏈霉親和素。
Excitation Laser：Blue Laser (488 nm)
一種綠色熒光標記的鏈霉親和素，吸收波長為488nM，發(fā)射波長為510nM。
Description：
Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.
應(yīng)用簡介：
Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.
基本數(shù)據(jù)：
Molecular Weight ～60 kDa (tetramer)
Concentration 5 mg/ml
Modification level 2.8 FITC/tetramer
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鏈霉親和素其他相關(guān)產(chǎn)品：
Streptavidin-FITC
Streptavidin-TRITC
Streptavidin-CY5
Streptavidin-CY3
Streptavidin-thiol
FITC-BSA
TRITC-BSA
BAS-CY5
BAS-CY3
BAS-Biotin
Concanavalin A-CY5
Concanavalin A-CY3
Concanavalin A-CY5.5
Concanavalin A-FITC
Concanavalin A-Biotin
Concanavalin A-SH
Concanavalin A-TRITC
鏈霉親和素(SA)簡述：
鏈霉親和素(SA)是Streptomyces avidinii菌的分泌物，其分子量及結(jié)合生物素的能力與雞蛋清中的親和素相似，等電點6.0，非特異性結(jié)合遠比親和素低。
鏈霉親和素(SA)的來源：
SA 是Streptomyces avicllrdi菌培養(yǎng)過程中的分泌產(chǎn)物，主要通過2一亞氨基生物索親和層析法提純，1L培養(yǎng)液中含蛋白10~60mg。鏈霉親和素是四聚體蛋白，大小為66KDa。一分子鏈霉親和素可以高度特異性地與四分子生物素結(jié)合，兩者之間的親和力極為強烈。
Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.
