Anti-Bcl-2抗體現(xiàn)貨 Anti-Bcl-2抗體說明書

Anti-Bcl-2抗體說明書

From:ABCAM

Catalog Number : RS- 0032R

Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent )

Relevance: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

.S Anti-Bcl-2抗體說明書pecificity : Anti- Bcl-2 is a mouse monoclonal antibody

specific for Bcl-2ofchicken

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype: IgG
• mol wt: 26kDa
• Application :
• WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 KLH conjugated synthetic peptide derived from human Bcl-2 C-terminus
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user..

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃ Anti-Bcl-2抗體說明書

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.