激情综合啪啪6月丁香,久久久久国产精品91福利,99精品日韩欧美在线观看,91成人午夜福利在线观看国产

行業(yè)產(chǎn)品

  • 行業(yè)產(chǎn)品

廈門慧嘉生物科技有限公司


當(dāng)前位置：廈門慧嘉生物科技有限公司>>ELISA試劑盒 >>小鼠的ELISA試劑盒 >>優(yōu)品質(zhì) 小鼠（IL-3）ELISA試劑盒 小鼠白細(xì)胞介素-3（IL-3）ELISA試劑盒

優(yōu)品質(zhì) 小鼠（IL-3）ELISA試劑盒 小鼠白細(xì)胞介素-3（IL-3）ELISA試劑盒

返回列表頁
參  考  價面議
具體成交價以合同協(xié)議為準(zhǔn)

產(chǎn)品型號

品       牌

廠商性質(zhì)代理商

所  在  地廈門市

聯(lián)系方式：陳先生查看聯(lián)系方式

更新時間：2018-05-23 11:19:11瀏覽次數(shù)：584次

聯(lián)系我時，請告知來自 環(huán)保在線

產(chǎn)品簡介

廈門慧嘉生物公司*經(jīng)營小鼠白細(xì)胞介素-3(IL-3)ELISA試劑盒\(zhòng)Santa\Abcam\Cst\jackson \Pierce\Sigma\Amresco\Qiagen\Cayman\abnova\millipore\invitrogen\ merk \ ebioscience \prospec\peprotech細(xì)胞因子*

詳細(xì)介紹

 廈門慧嘉生物*專業(yè)銷售ELISA試劑盒\(zhòng)Santa \ Abcam \ Cst \ jackson \ Pierce \ Sigma \ Amresco \Qiagen \ Cayman \ abnova \ millipore \ invitrogen \ merk \ ebioscience \prospec \ peprotech細(xì)胞因子*。等生物試劑產(chǎn)品。實驗為大，誠信經(jīng)營，為客戶提供“zui高質(zhì)量的產(chǎn)品”和“的服務(wù):  : 或登陸http://www.biohj.com（向客服人員索取原版說明書）。歡迎廣大老師來詢！”

小鼠白細(xì)胞介素-3（IL-3）ELISA試劑盒
小鼠白細(xì)胞介素-4（IL-4）ELISA試劑盒
小鼠白細(xì)胞介素-5（IL-5）ELISA試劑盒
小鼠P選擇素（P-Selectin/CD62P）ELISA試劑盒 
小鼠神經(jīng)生長因子（NGF）ELISA試劑盒
小鼠神經(jīng)營養(yǎng)因子3（NT-3）ELISA試劑盒
小鼠神經(jīng)營養(yǎng)因子4（NT-4）ELISA試劑盒
小鼠骨保護(hù)素（OPG）ELISA試劑盒
小鼠B因子（BF）ELISA試劑盒
小鼠腫瘤壞死因子α（TNF-α）ELISA試劑盒 
小鼠腫瘤壞死因子β（TNF-β）ELISA試劑盒
小鼠Ⅳ型膠原（Col Ⅳ）ELISA試劑盒
小鼠己糖激酶（HK）ELISA試劑盒
小鼠丙酮酸脫氫酶E1（PDH E1）ELISA試劑盒
小鼠糖原合成酶激酶（GSK）ELISA試劑盒
小鼠羥*（Hyp）ELISA試劑盒
小鼠骨保護(hù)素配體（OPGL）ELISA試劑盒
小鼠乳鐵傳遞蛋白/乳鐵蛋白（LF/LTF）ELISA試劑盒
小鼠色素上皮衍生因子（PEDF）ELISA試劑盒
小鼠腎損傷分子1（Kim-1）ELISA試劑盒 
小鼠糖皮質(zhì)類固醇受體（GR）ELISA試劑盒
小鼠黃體生成素釋放激素（LHRH）ELISA試劑盒
小鼠α半乳糖基抗原（α-Gal）ELISA試劑盒 
小鼠β氨基己糖苷酶A（β-Hex A）ELISA試劑盒 

檢測范圍：

Request Information

靈敏度：

Request Information

反應(yīng)時間：

1-3h

所需樣本體積：

50-100ul

檢測波長：

450 nm

說明書：

（向客服人員索取原版說明書）

原理 ：

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase （HRP） is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

特異性：

This assay has high sensitivity and excellent specificity for detection of Rat ACHE. No significant cross-reactivity or interference between Rat ACHE and analogues was observed.

精密度：

Intra-assay Precision （Precision within an assay）: CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision （Precision between assays）: CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

樣本搜集及儲存：

Serum: Use a serum separator tube （SST） and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediay or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.

檢測步驟：

Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody （1x） to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. （Biotin-antibody （1x） may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.）
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer （200μl） using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin （1x） to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

結(jié)果計算：

Using the professional soft "Curve Exert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic （4-PL） curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ACHE concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

 

廈門慧嘉生物*專業(yè)銷售ELISA試劑盒\(zhòng)Santa \ Abcam \ Cst \ jackson \ Pierce \ Sigma \ Amresco \Qiagen \ Cayman \ abnova \ millipore \ invitrogen \ merk \ ebioscience \prospec \ peprotech細(xì)胞因子*。等生物試劑產(chǎn)品。實驗為大，誠信經(jīng)營，為客戶提供“zui高質(zhì)量的產(chǎn)品”和“的服務(wù)”:  : 或登陸http://www.biohj.com（向客服人員索取原版說明書）。歡迎廣大老師來詢！


您感興趣的產(chǎn)品PRODUCTS YOU ARE INTERESTED IN

環(huán)保在線 設(shè)計制作，未經(jīng)允許翻錄必究 .? ? ? Copyright(C)?2021 http://www.hg1112.cn,All rights reserved.

以上信息由企業(yè)自行提供，信息內(nèi)容的真實性、準(zhǔn)確性和合法性由相關(guān)企業(yè)負(fù)責(zé)，環(huán)保在線對此不承擔(dān)任何保證責(zé)任。 溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買產(chǎn)品前務(wù)必確認(rèn)供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

請 登錄 后再收藏

提示

您的留言已提交成功！我們將在第一時間回復(fù)您~
三级无码日B视频| 涩涩屋操美女视频| 男人把昆吧放女人屁股里| 国奴精品毛片av一区二区三区| 中文字幕av一区二区三区哈| 色噜噜在线一区二区三区| 亚洲高清中文字幕一区二区三区| 啊啊不要你那痛死爽死了直播一区| 午夜无码a级毛片| 国产精品碰碰现在自| 中文字幕你懂的av一区二区| 操女人逼逼骚逼逼| 粉色av一区二区三区| 大男人在线无码直播| 精品福利一区二区三区在线观看| 天天日天天干天天天天操| 美国女人抠插bbb| 日韩欧美综合一二三区| 国产亂倫近親相姦| 91秦先生全集在线观看| 国产综合精品一区二区青青| 日本最新免费不卡一区二区三区| 亚洲女同一区二区三久久精品| 亚洲精品自拍偷拍| 女人张开腿让男人捅个爽| 日韩欧美一区二三区风间由美| 搬开女人下面使劲插视频| 鸡巴插进女人的逼里| 欧美一区二区三区身体| 亚洲一级片在线播放| av在线国产哟哟| 免费男人和女人黄片| 伊人久久丁香色婷婷啪啪| 开心五月播五月亚洲第一| 熟女菊蕾老妇俱乐部视频| 欧美日韩亚洲人人夜夜澡| 男人把昆吧放女人屁股里| 差鸡巴没码在线观看| 大鸡吧视频在线观看| 两人爽爽爽无码免费视频| 少妇无套带白浆嗯呢啊污|