人*脫氫酶(GDHGLDH)檢測試劑盒英文名稱Human Glutamate dehydrogenase;GDHGLDH ELISA Kit齊一生物科技(上海)有限公司試劑種類眾多:覆蓋分析化學(xué)品,無機(jī)化學(xué)品,有機(jī)化學(xué)品,生物化學(xué)品,材料化學(xué)品.試劑信息齊全:包含試劑特性描述,詳細(xì)質(zhì)量信息,應(yīng)用方法、標(biāo)準(zhǔn),用途以及正在開發(fā)的前沿應(yīng)用
人*脫氫酶(GDHGLDH)檢測試劑盒elisa技術(shù)流程
ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性.在測定時,受檢標(biāo)本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng).用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開.再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上.此時固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例.加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測定方法達(dá)到很高的敏感度.ELISA可用于測定抗原,也可用于測定抗體.
然而,影響Elisa試驗結(jié)果的因素很多,故加強(qiáng)各個環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點.
試驗原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將*標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
原理
免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).
1026901 MagAttract Suspension G (13/15),KG 13 ml volume of suspension G
9233576 Label, Biorobot3000
9019229 Memory Card, PyroQ24
9019215 Cog belt kit - Robot, PyroQ96MD
9019214 PCB - Shutter, PyroQ96MD
9019208 Cable - Y robot, PyroQ96MD
1066850 AIV-H7 RT-PCR REAGENT LE
9232904 YZ-CPU Board
1061082 CT PCR REAGENT I
1061107 PG AIV-H9 RT-PCR Assay (48) Z
1061112 HIV-1 RT-PCR REAGENT I
1061114 AIV-H7 RT-PCR REAGENT
9016652 Vacuum Trap Bottle 4l, UTP
9016420 Tubing Sys., Bottle, BR8 RD UTP
9016271 Maintenance Kit, BR MDx v2
9016268 Upgrade Kit, DISU, BR8000
9016237 Bag for Waste Container
9016122 Maintenance Kit, RoboVac, BR8000 I,SBS
9019176 Window, PyroQ96MD
9011840 Set, Tubing, PeristalticPump, In/Out, P3
9019137 Reagent chamber lid, PyroQ96ID
9019120 PCB 5 - Connection, PyroQ96ID
9019111 PCB - Interconnection, PyroQ96ID
9019084 PyroMark Q96 ID Software (5) For use with PyroMark Q96 ID instrument.Software for setting up a run and analyzing mutations and sequence identification.
9019063 PyroMark Q24 MDx Software For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
9019020 Coupling, Z axis, EZ1xl
9019019 Coil, EZ1xl
9019005 Door lock assembly, EZ1xl
9022710 Enclosure, Silver, DML3000
9016069 Maintenance Kit, RoboVac, BR8000 II
9016040 Modules, DISU, BR8000 v3.0
9016000 Magnet head for PCR plateMagnet head for
9015964 Z-drive unit, BR8000 v1.1
9015635 Fuse-1, 6.3A (for PSB board), M48
9015589 EZ1 DNA Buccal Swab Card Pre-programmed card for BioRobot EZ1 DNA Buccal Swab Protocol
9015585 EZ1 DNA BLD Card Pre-programmed card for BioRobot EZ1 DNA Blood 200 l and 350 l Protocols
9015548 Pulley Overhead, BR8, RD v1.1
9015539 Liner, worktable
9015535 Cover, front, type C
9015530 Power supply unit, complete
9241261 Full Agreement Depot Repair, PC, 1 yr "The Full Agreement Depot Repair, PC provides full service coverage for the PC and monitor used in the digene HPV application. The service is conducted as an instrument exchange service.
All shipping, labor and repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."
9019158 PCB 5 - Connection, PyroQ96MD
9014184 Upgrade kit, LabHandT-RapidPlate
9014183 ZLV Z-Modul; Washable Probe 0,9mm;BR8000
9021190 Adapter, 96-well MP, BS96
9021107 Skin Panel, PC Mount Switch Mount, DCU
9021084 PLC Module, MES, DCU
9020992 Shaker Assembly, DCU
9020926 Lid, QXT
9020923 Slideout electrical tray, SMC, QGY
9016362 EZ1 DNA BACT Card
9011926 Plate holder, long
9236778 Elution Microtube Adapter, SBS