齊一生物胃泌素細(xì)胞抗體（GCA）檢測試劑盒英文名稱Human gastrin cell antibody,GCA ELISA Kit齊一生物科技（上海）有限公司試劑種類眾多：覆蓋分析化學(xué)品,無機(jī)化學(xué)品,有機(jī)化學(xué)品,生物化學(xué)品,材料化學(xué)品.試劑信息齊全：包含試劑特性描述,詳細(xì)質(zhì)量信息,應(yīng)用方法、標(biāo)準(zhǔn),用途以及正在開發(fā)的前沿應(yīng)用

齊一生物胃泌素細(xì)胞抗體（GCA）檢測試劑盒elisa技術(shù)流程
ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性,酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性,又保留酶的活性.在測定時,受檢標(biāo)本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應(yīng).用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開.再加入酶標(biāo)記的抗原或抗體,也通過反應(yīng)而結(jié)合在固相載體上.此時固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例.加入酶反應(yīng)的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進(jìn)行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應(yīng)的結(jié)果,使測定方法達(dá)到很高的敏感度.ELISA可用于測定抗原,也可用于測定抗體.
然而,影響Elisa試驗結(jié)果的因素很多,故加強(qiáng)各個環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點.
試驗原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測.先將*標(biāo)記的抗體同時溫育.洗滌后,加入親和素標(biāo)記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關(guān)系.
原理
免疫測定技術(shù)的基礎(chǔ)在于抗原抗體之間的特異結(jié)合反應(yīng),所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術(shù)得到的單克隆抗體,而抗原或抗體的標(biāo)記物如酶標(biāo)結(jié)合物則通過各種化學(xué)合成方法制備.近年來,隨著分子生物學(xué)的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).
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9241217    RCS, Premium Agreement, 2PM, 1 yr    "The Premium Cover Agreement RCS, 2 PM provides full service coverage for the Rapid Capture System (on-site repair service) and the associated DML, the MST II Vortexer, and the PC (in case of an error the associated equipment is exchanged).

Costs for services are fully covered including shipment, travel, labor, and parts. The agreement also includes two annual on-site preventive maintenance visits, with costs for labor and parts covered. With a Premium Agreement, there is a priority response time for the on-site service of 24 hours (1 working day)."
9238818    Rect. carrier for 4 Falcon tubes 15ml    
9233335    Valve, pressure relief, dispenser,BR8000    
9233291    Magnet, stop position, cover, BR8000    
9237715    BR M48, Full Agreement    The Full Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9237713    EZ1, Installation    The Installation, BR EZ1 facilitates the quick implementation of the BioRobot EZ1 or EZ1 Advanced into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of the BioRobot EZ1 or EZ1 Advanced instrument and provides the user with an introductory training on operation and user maintenance. The installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
9233165    PCB, ReaderPowerSupply, X-main    
9237159    Laser Driver, Uniphase    
9237149    XYP Carrier    
9237116    Upgrade App., Ni-NTA Superflow    
9237101    Upgrade, BR plus 1 ext. device    Upgrade BioRobot with one approved integration device
9236365    Filter Fan XYP    
9232932    Reader(VIS), with auto plate positioning    
9232931    Reader Verdrahtung Netz-Mainboard Plato7    
9236350    Pressure Regulator, 10 Psi    
9236327    PMT    
9236300    Gasket, auto vac, top BR8000 v1.00    
9230316    Container, SystemLiquid, 10 l    
9230312    Power cables for 4 syringe pumps    
9235273    Bottle, condensate, auto vac, BR8000    
9230280    Cable, card-X to bar-code camera Denso    
9230058    Toothwheel, Z-Motor, P1/3 / BR3/96    
9234860    Tubing coupling piece for system liquid    
9238666    Pneumatic spring, 200 Nm    
9238661    Filter dryer    
9238660    Temperature sensor    
9242029    QIAxcel, Repair Fee    "Repair service delivery send to Regional Repair Center
Service response time (repair) 7-10 days turnaround time
Periodic inspection/maintenance: 0
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9241895    QIArent&go - Rent out fee QIAsymphony AS    
9241814    Pyro Q24, PM Agreement    An agreement for preventive maintenance provides coverage for travel, labor, and parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.
9241165    Board, smart key, QX    
9019855    72-well High Profile Locking Ring    
9019833    Cable, RS232,  external, V2, QX    
9019763    Motor, Y, V2, QX    
9019732    Board, LED scan, V2, QX    
9013659    BGR DT Adapter 1100ul P3xxx    
9013444    Reagent holder, 3-trough 20 ml    
9013437    Maintenance kit,  annual, BR9600 (F)    
9013426    Maintenance Kit BR 9600 (C)    
9019661    Manometer, PyroQ96VW    
9019659    Screw set VPT, PyroQ96VW    
9019654    Springloaded pins VPT, PyroQ96VW    
9019620    Belt, Z-axis assembly, 1 ch., Cx2    
9019606    Pipettor, Piston, Cx2    
9019576    Opto switch, Y-axis, Cxx    
9019527    PLATE SEPARATOR 96 WELL WHITE    
9019525    UV support frame, QIA    
9019519    ZLV ADAPTER KIT HIGH SKIRT    
204143    QuantiTect SYBR Green PCR Kit (200)    For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect SYBR Green PCR Master Mix,* 2 x 2.0 ml RNase-free water
9019438    Fan 115VAC   50/60 Hz  0.26A    
9019409    Sensor, rotor timing & home, RG3    
9019407    Switch, mains voltage selector, RG3    
9019400    Sensor, infrared, RG3    
9019361    Fan, Base, 12V 120mm, RG6    
9019354    Coupling, rotor shaft, RG6    
1027299    AllStars Cell Death Contr.siRNA (20nmol)    
9019340    LED, blue (470), RG6    
9019236    Bearing, PyroQ24    
9020462    PMT, GSC    
1026901    MagAttract Suspension G (13/15),KG    13 ml volume of suspension G