人肝脂酶（HL）檢測試劑盒英文名稱Human hepatic lipase,HL ELISA Kit齊一生物科技（上海）有限公司新品*歡迎您訪問我們的如有什么問題請給我們或者留言741653262,告訴我您的需要,并留下您寶貴的意見和建議

人肝脂酶（HL）檢測試劑盒elisa技術流程
ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記.結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學活性,酶標記的抗原或抗體既保留其免疫學活性,又保留酶的活性.在測定時,受檢標本（測定其中的抗體或抗原）與固相載體表面的抗原或抗體起反應.用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開.再加入酶標記的抗原或抗體,也通過反應而結(jié)合在固相載體上.此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例.加入酶反應的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關,故可根據(jù)呈色的深淺進行定性或定量分析.由于酶的催化效率很高,間接地放大了免疫反應的結(jié)果,使測定方法達到很高的敏感度.ELISA可用于測定抗原,也可用于測定抗體.
然而,影響Elisa試驗結(jié)果的因素很多,故加強各個環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學的優(yōu)點.
試驗原理
試劑盒是固相夾心法酶聯(lián)免疫吸附實驗（ELISA）.已知濃度的標準品、未知濃度的樣品加入微孔酶標板內(nèi)進行檢測.先將*標記的抗體同時溫育.洗滌后,加入親和素標記過的HRP.再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用.產(chǎn)生顏色.顏色的深淺和樣品中的濃度呈比例關系.
原理
免疫測定技術的基礎在于抗原抗體之間的特異結(jié)合反應,所以任何優(yōu)質(zhì)的診斷試劑離不開優(yōu)質(zhì)的原料,如抗原抗體,酶等.以前用于免疫測定的抗原通常為各種純化抗原,而抗體則為純化抗原免疫動物后獲得的多克隆抗體和使用雜交瘤技術得到的單克隆抗體,而抗原或抗體的標記物如酶標結(jié)合物則通過各種化學合成方法制備.近年來,隨著分子生物學的發(fā)展,使用基因工程方法制備各種特殊的抗原或抗體及其酶結(jié)合物等免疫測定試劑幾成為現(xiàn)實的新一代試劑,各種新型使用的測定方法也不斷出現(xiàn).
人軟骨寡聚蛋白(COMP)ELISA試劑盒
人多藥耐藥相關蛋白(MRP)ELISA試劑盒
人高速泳動蛋白17(HMG-17)ELISA試劑盒
人空泡毒素相關蛋白A(VacA)ELISA試劑盒
人玻連蛋白/體外粘連蛋白(VN/CD51+CD61)ELISA試劑盒
人波形蛋白(VIM)ELISA試劑盒
人表面活性蛋白D(SP-D)ELISA試劑盒
人阿拉伯半乳糖蛋白(AGPs)ELISA試劑盒
人β乳球蛋白(β-Lg)ELISA試劑盒
人β連環(huán)蛋白/聯(lián)蛋白(β-Cat)ELISA試劑盒
人淀粉樣前體蛋白(βAPP)ELISA試劑盒
人β2糖蛋白(β2-GP)ELISA試劑盒
人α乳清蛋白(α-La)ELISA試劑盒
人N-乙酰基-絲氨酰-天門冬酰-賴氨酰-*(AcSDKP)ELISA試劑盒
人的牛血清白蛋白殘留檢測ELISA試劑盒
人β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒
人髓磷脂堿性蛋白(MBP)ELISA試劑盒
人結(jié)蛋白(Des)ELISA試劑盒
人S100蛋白(S-100)ELISA試劑盒
人巨噬細胞炎性蛋白2(MIP-2)ELISA試劑盒
人凝膠原蛋白(gelson)ELISA試劑盒
人踝蛋白(talin)ELISA試劑盒
人角化細胞內(nèi)分泌因子(KAF)/雙調(diào)蛋白(AR)ELISA試劑盒
人β內(nèi)啡肽(β-EP)ELISA試劑盒
人皰疹病毒6型抗體（IgM)ELISA試劑盒
人25羥*3(25(OH)D3)ELISA
人1,25二羥基*3(1,25-(OH)2D3/DVD/DHVD 3)ELISA
人水通道蛋白4（AQP-4-AB）抗體elisa
"人Ⅰ型膠原N末端肽(NTX)ELISA試劑盒
"
人硫化氫（H2S）ELISA試劑盒
人路氏錐蟲（ELISA）
人正五聚蛋白3(PTX3）ELISA試劑盒
人鈣衛(wèi)蛋白(calprotein)ELISA試劑盒
人抑制素B(INHB)Elisa
（人血清）新型隱球菌抗原CNAgELISA
人VEGFR4
9238836    BioSprint96, Basic Agreement, no PM    "Repair service delivery: onsite
Service response time (repair): 5 business days
Periodic inspection/maintenance: not included
Inspection/maintenance delivery: not applicable
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: not applicable"
9241211    Full Agreement Depot Repair, DML2000, 1y    "The Full Agreement Depot Repair, DML2000 provides full service coverage for the DML 2000 luminometer. The service is conducted as an instrument exchange service. 
All shipping, labor and repair part costs related to an instrument exchange are included for 1 year. Instrument exchange time is 2 - 3 working days."
9241191    Magnet, tool, QX    
9238825    Spare bucket for plate rotor    
9238815    Rect. carrier for 12 tubes 10 ml w/ cap    
9019472    TAPE SPACER CLEAR GS    
9019464    UV tube, Cxx    
9243650    Comprehen Valid., EZ1/QIAcube/QIAsymph    """The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to complete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and complete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan encompasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via ) any validation data requested after the on-site visit. A QIAGEN specialist will complete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring compliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (/CD/flashdrive) to the laboratory. The laboratory is responsible for final compilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating compliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following completion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """
9237333    Install, BR plus 3 integr. w. scheduling    Installation and training, BioRobot System including three approved integration devices with scheduling
9237328    Upgrade, BR & 2 ext. devices w. sched.    Upgrade BioRobot with two approved integration devices using scheduling software
9237173    Shield, V-housing    
9233208    Tubing, liquid system 2.5/1.5    
9237155    V-Housing with Sample Needle    
9237150    Heater Top    
9016151    #App. Package, M48, Pathology    
9019179    Opto - plate lock closed, PyroQ96MD    
9011883    Upgrade, high speed pipetting system    
9011882    Back unit, BR9600    
9011823    Z-Modul; Tip-Adapter 300μl; BR8000    
9236916    3 Year Warranty, LiquiChip    LiquiChip 3-year Warranty with 1 Preventive Maintenance per year.  Included in system price.  This warranty must be included as a line item on the system quote.  It covers the system for three years total including all per incident travel and labor charges, all repair parts, one preventive maintenance visits per year (three in total), and 48- hour priority response.
9019143    Power supply, PyroQ96    
9019129    Cable - Reagent, PyroQ96ID    
9019126    PCB 2 - Connection, PyroQ96ID    
9019114    IPC mainboard, PyroQ96ID    
9019025    Worktable, side wall, left, EZ1xl    
9019012    Motor Y, EZ1xl    
9018942    Adapter, 2xRing of 12 COBAS Amplicore    
9022737    Rotor-Gene AssayManager SOW    
9016061    Tubing Set, Syringe inlet, BR8000 I    
9015823    CD, Field Syringe Calibration, BR8000    
9015690    Rings (10), pinching, 4.5/3.2 mm    
9015599    CD, Manual, Service, BIOROBOT 8000 II    
9015587    EZ1 DNA Buffy Coat Card    Pre-programmed card for BioRobot EZ1 DNA Buffy Coat Protocol
9019166    Lead screw - X, PyroQ96MD    
9019153    PCB - Robot Control, PyroQ96MD    
9014152    Top, Robovac, BR8 RD v4.2    latest version of robovac top.
9014027    Belt,Y-drive,Fixation Upgrade Kit,BR8000    
9013868     Tip-Tray Holder, 300 μl tips    Holder for accommodating a tip tray containing 96 disposable tips of 300 μl capacity
9021099    Skin Panel, Input Drawer, DCU    
9021003    Cable, Y Drive, DCU    
9020939    Vacuum Pump, incl. trap and filters, QXT    
9020936    Kit, calibration, mech. variation, RGQ    
9020925    Packaging (complete), SMC, QXT    
9020912    Lid, SMC, QGY    
9020697    Fan, electrical, 80mm, C1x    
9020678    Rail, centre, CX1    
9020394    Sensor, induct, drawer, AS, Qsym    
9020364    Assay out drawer, right, AS, Qsym    
9020343    Cable - Serial, RGQII    
9020284    Accessory, cooling block insert, Cx2    
9020258    Side Pannel, top left, Qsym    
9020229    Slideout electrical tray, QXT    
9240522    Motor Assy, Reagent Pump, AP98    
9240519    Motor Assy, Capper U/D, AP98    
9020022    Lumicheck Plate Service Package, DML3    
9238890    PCB, UI-01, BS96    
9238889    PCB, MM-01, BS96    
9241217    RCS, Premium Agreement, 2PM, 1 yr    "The Premium Cover Agreement RCS, 2 PM provides full service coverage for the Rapid Capture System (on-site repair service) and the associated DML, the MST II Vortexer, and the PC (in case of an error the associated equipment is exchanged).

Costs for services are fully covered including shipment, travel, labor, and parts. The agreement also includes two annual on-site preventive maintenance visits, with costs for labor and parts covered. With a Premium Agreement, there is a priority response time for the on-site service of 24 hours (1 working day)."
9238818    Rect. carrier for 4 Falcon tubes 15ml    
9233335    Valve, pressure relief, dispenser,BR8000    
9233291    Magnet, stop position, cover, BR8000    
9237715    BR M48, Full Agreement    The Full Agreement M48 provides full service coverage for the BioRobot M48. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9237713    EZ1, Installation    The Installation, BR EZ1 facilitates the quick implementation of the BioRobot EZ1 or EZ1 Advanced into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of the BioRobot EZ1 or EZ1 Advanced instrument and provides the user with an introductory training on operation and user maintenance. The installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
9233165    PCB, ReaderPowerSupply, X-main    
9237159    Laser Driver, Uniphase