人雄烯二酮（ASD）檢測(cè)試劑盒Human Androstenedione,ASD ELISA Kit齊一生物科技有限公司新品*，齊一生物科技有限公司*經(jīng)營(yíng)大鼠ELISA試劑盒,人elisa試劑盒，小鼠elisa試劑盒等種屬試劑盒，歡迎您訪問我們的，www.qiyibio。。ｃｏｍ全國(guó)：歡迎選購(gòu)！

人雄烯二酮（ASD）檢測(cè)試劑盒類別：酶聯(lián)免疫分析檢測(cè)試劑盒
規(guī)格：96T/48T
包裝：盒
檢測(cè)原理：采用雙抗夾心法
保質(zhì)期：六個(gè)月
產(chǎn)品保存條件：2-8°C
應(yīng)用范圍：僅供科研研究實(shí)驗(yàn)使用
樣品形式：血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體（具體情況請(qǐng)咨詢）
標(biāo)本的采集及保存：
1.血清：室溫血液自然凝固10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存  過程 中  如有沉淀形成，應(yīng)再次離心。
2.血漿：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑，混合10-20分鐘后，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。
3.尿液：用無(wú)菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清：檢測(cè)分泌性的成份時(shí)，用無(wú)菌管收集。離心20分鐘左右（2000-3000轉(zhuǎn)/ 分）。仔細(xì)收集上清。  檢測(cè)細(xì)胞內(nèi)的成份時(shí)，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞濃度達(dá)到100萬(wàn)/ml左右。通過反復(fù)凍融，以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。
5.組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的PBS，PH7.4。用液氮迅速冷凍保存?zhèn)溆谩?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS（PH7.4），用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。分裝后一份待檢測(cè)，其余冷凍備用。
標(biāo)本要求： 
1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融
2．不能檢測(cè)含NaN3的樣品，因NaN3抑制辣根過氧化物酶的（HRP）活性。
Consumable    59824    EpiTect Fast DNA Bisulfite Kit (50)
Instrument Part    9011422    Tip Rack Holder, RCS1
Instrument Part    9235701    Cable 5V, RCS1
Consumable    1020952    Protease Resuspension Buffer (6/15),KG
Instrument Part    9015095    Vacuum Manifold Top, SBS, RD3
Instrument Part    9238620    Computer board Zent3, no Eprom
Instrument Part    9238630    Jumper, 2-channel
Instrument Part    9238657    Main cable, centrifuge
Consumable    204741    QuantiTect Multiplex PCR NoROX Kit (40)    "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect Multiplex PCR NoROX Master Mix, 2.0 ml RNase-free water

For quantitative, real-time PCR and two-step RT-PCR using probe detection with multiple assays."
Instrument Part    9016068    Maintenance Kit, Basic, BR8000 II
Instrument Part    9016685    Washer Mechanical, RCS1
Instrument Part    9018949    Adapter, Tip Rack Holder for 96-Tip Rack
Consumable    204374    Rotor-Gene Probe PCR Kit (400)    The Rotor-Gene Probe PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time PCR and two-step RT-PCR using sequence-specific probes.
Consumable    1054575    HiSpeed Plasmid Giga EF Kit (5)
Instrument Part    9019109    IPC bus backplane, PyroQ96
Instrument Part    9238684    Eprom and controller 4-15, Zent 3C
Instrument Part    9238709    Key with ring
Instrument Part    9238768    Swing out rotor for 4 buckets    Sigma Rotor # 11140
Instrument Part    9238796    Rectangular bucket
Instrument Part    9238805    Round sealing cap, clear
Instrument Part    9230932    SlidingSheet, back, 12-Samtrack
Customized Kit    1036685    HiPerFect Transfection Reagent (50 ml)
Consumable    239901    GelPilot DNA Loading Dye,5x (6 x 500μl)
Instrument Part    9016487    Cable REDGE/Gateway, RS232, Kloehn
Consumable    129115    Nuclease-Free Water (1000 ml)
Instrument Part    9238751    Glases, Laser protection, 530-650nm
Instrument Part    9238622    Main board Zent3, no Eprom (>SN85353)
Instrument Part    9238633    Grey wedge (long)    " This is a thin plastic sheet with a printed gradient of dots that is held by a bracket on the motor so that it is situated in the u shape of the sensor. 

SIGMA sensor"
Instrument Part    9238655    Computer board cable, 3-channel
Instrument Part    9016566    Syringe Pump, RCS1
Instrument Part    9018955    Reagent Block, 24x1.5ml Tapered-Base
Consumable    204174    Rotor-Gene SYBR Green RT-PCR Kit (400)    The Rotor-Gene SYBR Green RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time one-step RT-PCR using SYBR Green I.
Instrument Part    9018857    Adapter Plate, Pres. Sensor, BR9604/3000
Consumable    76554    RNAprotect Animal Blood Tubes 500μl (50)
Consumable    129916    QIAGEN RNase Inhibitor (500 reactions)    "QIAGEN RNase Inhibitor is a protein of nonhuman origin that binds noncovalently to an inactivates a variety of ribonucleases (e.g., RNase A, B, and C). It does not interfere with RNase T1,T2, H, U1, U2, or CL3. QIAGEN RNase Inhibitor is a polyclonal antibody which is highly active and inhibits more than 90% of RNase activity, whereas HPRI (human placental RNase inhibitor) inhibits only 50%. For this reason, it can be used at lower concentrations compared to other RNase
inhibitors. In addition, QIAGEN RNase Inhibitor is stable over a wide pH and temperature range and at varying dithiothreitol (DTT) concentrations. QIAGEN RNase Inhibitor is supplied at a concentration of 4 units per microliter in 2 mM KH2PO4, 8.0 mM Na2HPO4, 3.0 mM KCl, 150 mM NaCl, pH 7.4, and 50% glycerol."
Instrument Part    9019138    Lens array, PyroQ96ID
Consumable    4692163    RespiFinder RG Panel CE
MDx    4504263    artus Parvo B19 RG PCR Kit (24) CE
MDx    4559263    artus C.trachomatisPlus RG PCR Kit(24)CE
Instrument Part    9238663    Hook, motorized lid lock
Instrument Part    9015521    Interlock switch, for maintenance
Instrument Part    9015725    Probe, 0.4 mm, BR8000 v1.01
Instrument Part    9016569    Power Supply, RCS1
Consumable    204574    Rotor-Gene Probe RT-PCR Kit (400)    The Rotor-Gene Probe RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly sensitive quantification in real-time one-step RT-PCR using sequence-specific probes.
Consumable    204363    QuantiTect Probe PCR + UNG Kit (200)    The supplied, ready-to-use master mix contains HotStarTaq DNA Polymerase, ROX passive reference dye, and a dNTP mix in an optimized buffer. The dNTP mix includes dUTP, allowing optional treatment with UNG. The UNG enzyme is provided separay. For convenience, the master mix can be stored at 2–8°C.
Instrument Part    9241776    QIAgility, PM Service    "- The inspection/preventive maintenance (PM) service is an on-site equipment service, performed by a QIAGEN service specialist
- Service covers all travel and labor costs plus all necessary parts to perform an inspection/PM service 
- All required testing material and certified equipment is provided by the service specialist
- During the inspection/PM procedure, all respective components of the equipment are inspected and tested to ensure instrument is performing according to specifications  
- The instrument will also be thoroughly checked and inspected to determine if any additional parts and services are required to get the instrument running to its optimal performance. There will be an additional charge for such parts and services if any are needed
- Test results are documented in a GMP/GLP-compliant visit report and accessible for the customers
- Pro-active notification of the next Inspection/PM due will be organized by QIAGEN"
Instrument Part    9019112    PCB - Controller, PyroQ96ID
Instrument Part    9019119    Cog belt kit - X/Y, PyroQ96ID
Instrument Part    9019145    Fan - rear 60x60, PyroQ96MD
Instrument Part    9019169    Fan - peltier 60x60, PyroQ96MD
Instrument Part    9019968    Accessories for balance, IQ/OQ, QS
Consumable    929563    QX Size Marker 250 bp - 8 kb v2.0(50 μl)
Consumable    929559    QX Size Marker 100 bp - 2.5 kb (50 μl)
Consumable    208054    QuantiNova SYBR Green PCR Kit (500)
Consumable    51604    QIAamp Fast DNA Stool Mini Kit (50)
Accessory    69975    TissueLyser 5 mm Bead Dispenser, 96-well    For dispensing 96 beads (5 mm diameter) in parallel
Consumable    61104    QIAamp DSP DNA Blood Mini Kit (50), CE        For 50 preps: QIAamp Mini Spin Columns, buffers, reagents, tubes, VacConnectors, and multi-language handbook