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精氨酸酶(ARG)檢測(cè)試劑盒

時(shí)間:2015/5/6閱讀:781
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精氨酸酶(ARG)檢測(cè)試劑盒

適用生物 Homo sapiens (Human,人)
精氨酸酶(ARG)檢測(cè)試劑盒檢測(cè)范圍 3.13-200ng/mL 靈敏度 1.34ng/mL
樣本類型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
實(shí)驗(yàn)時(shí)長(zhǎng) 4.5h 實(shí)驗(yàn)方法 雙抗夾心法 精氨酸酶(ARG)檢測(cè)試劑盒規(guī)格 96T

ELISA Kit for Arginase (ARG)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism speciesHomo sapiens (Human)
Product No.SEB120Hu
Sample typeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format96-well strip plate
Assay length4.5 hours
Detection range3.13-200ng/mL The standard curve concentrations used for the ELISA’s were 200ng/mL, 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL
SensitivityThe minimum detectable dose of this kit is typically less than 1.34ng/mL.

精氨酸酶(ARG)檢測(cè)試劑盒Specificity

This assay has high sensitivity and excellent specificity for detection of Arginase (ARG).
No significant cross-reactivity or interference between Arginase (ARG) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Arginase (ARG) and the recovery rates were calculated by comparing the measured value to the expected amount of Arginase (ARG) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)78-9791
EDTA plasma(n=5)89-9994
heparin plasma(n=5)93-10196

精氨酸酶(ARG)檢測(cè)試劑盒Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginase (ARG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginase (ARG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arginase (ARG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-105%90-99%90-101%91-101%
EDTA plasma(n=5)89-103%86-101%88-97%87-101%
heparin plasma(n=5)92-101%96-104%78-101%96-103%

精氨酸酶(ARG)檢測(cè)試劑盒Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120μLAssay Diluent A1×12mL
Detection Reagent B1×120μLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

精氨酸酶(ARG)檢測(cè)試劑盒Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50μL Stop Solution. Read at 450nm immediay.

精氨酸酶(ARG)檢測(cè)試劑盒Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase (ARG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginase (ARG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase (ARG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginase (ARG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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