β葡聚糖受體抗體詳細說明

【資料簡介】

相關(guān)標(biāo)記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin  Cy3   Cy5  
 Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC  
英文名稱    beta Glucan Receptor
中文名稱    β葡聚糖受體抗體
別    名    Dectin1; Dectin-1; Dectin 1; CLECSF12; C-type lectin domain family 7 member A; beta Glucan Receptor; Beta GR; BGR; C type calcium dependent carbohydrate recognition domain lectin superfamily member 12; Clec7a; CANDF4; DECTIN1; CLEC7A protein; Clecsf12; Dectin 1; DECTIN 1 receptor; Dectin1; Dendritic cell associated C type lectin 1; Lectin like receptor 1; beta Glucan Receptor; Putative transmembrane protein dectin 1; CLC7A_HUMAN.
研究領(lǐng)域    細胞生物  免疫學(xué)  淋巴細胞  
抗體來源    Rabbit
克隆類型    Polyclonal
交叉反應(yīng)    Human, Mouse, Rat, 
產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500（石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    28kDa
細胞定位    細胞膜 
性    狀    Lyophilized or Liquid
濃    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from human Dectin-1
亞    型    IgG
純化方法    affinity purified by Protein A
儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹    background:
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq].

Function:
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation (By similarity).

Subunit:
Homodimer. Interacts with SYK; participates in leukocyte activation in presence of fungal pathogens.

Subcellular Location:
Cell membrane; Single-pass type II membrane protein.

Tissue Specificity:
Detected in dendritic cells, in paracortical and medullary regions of lymph nodes, and in spleen red pulp and white pulp.

Post-translational modifications:
on tyrosine residues in response to glucan binding.

Similarity:
Contains 1 C-type lectin domain.

Gene ID:
64581

Database links:
Entrez Gene: 64581 Human
Entrez Gene: 56644 Mouse
Entrez Gene: 502902 Rat
Omim: 606264 Human
SwissProt: Q9BXN2 Human
SwissProt: Q6QLQ4 Mouse
Unigene: 143929 Human
Unigene: 239516 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

β-葡聚糖受體是一種非調(diào)理素依賴性受體,可在免疫反應(yīng)早期,在清除衰老細胞和防御感染方面發(fā)揮重要作用,進而實現(xiàn)其抗腫瘤、抗感染等生物學(xué)效應(yīng)。
產(chǎn)品圖片    
issue/cell: mo spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-DECTIN-1/CLECSF12/beta Glucan Receptor Polyclonal Antibody, Unconjugated (bs-2455R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining