Anti- GCS, FITC conjugated抗體說明書

【資料簡(jiǎn)介】

 Anti- GCS, FITC conjugated抗體說明書

Quantity size : 0.2ml （2mg/ml,Lyophilized. Buffer = 0.01M PBS, pH 7.4 with 1% BSA and 0.1% Sodium azide. Reconstitute with 0.2ml sterile distilled water. ）

 

Background: GCS （glucosylceramide synthase ） may serve as a 'flippase' as well as a glucosyltransferase that transfers glucose to ceramide. Able to use UDP-galactose to synthesize galactosylceramide with 10% of efficiency with which it utilizes UDP-glucose. [Catalytic activity] UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Lipid metabolism; sphingolipid metabolism. [Subcellular location] Endoplasmic reticulum membrane; Multi-pass membrane protein. Belongs to the glycosyltransferase 2 family.

 Anti- GCS, FITC conjugated抗體說明書

Specificity : · anti- GCS is a rabbit polycional antibody, FITC conjugated

           · specific for GCS of human, mouse and rat  

           · epitope mapping near the amino terminus of GCS of human, mouse and rat origin  

· Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG 

· mol wt：48kDa

Application : · FCM: 1:20-100

            · Immunohistochemistry : 1:20-1:200

            · Immunocytochemistry: 1:20-1:200

· Excitation spectrum: 490-495nm

· Emission spectrum: 520-530nm

· Optimal working dilutions must be determined by the end user.

· protect from light.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti- GCS, FITC conjugated抗體說明書