The kit is sued to assay the content of  Monkey Tuberculosis Antibody  in porcine serum, blood plasma, and other related Liquid samples.

 

The kit use ELISA  to assay Monkey Tuberculosis Antibody level in the sample，use Purified Monkey Tuberculosis an antigen to coat microtitration wells, make solid-phase an antigen, add Monkey Tuberculosis Antibody in coated microtitration, Combined With HRP  which labeled goat anti- Monkey antibody, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution and color develops, TMB becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, the depth of color and theMonkey Tuberculosis Antibody of sample were positively correlated, measure the optical densit （OD） at 450 nm with microtiter plate reader, calculate Monkey Tuberculosis Antibody concentration by standard curve.

 

1． extract as soon as possible after Specimen collection, Extracted according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can not be tested immediay, specimen can be kept in -20 ℃ to preserve, but repeated freezing and thawing should be avoided.

2． Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP activity of the horseradish peroxidase.

 

 

 

Negative control: sample OD< Calculate Critical（CUT OFF） isMonkey Tuberculosis Antibody Negative control.

Positive control: ample OD≥ Calculate Critical（CUT OFF） is Monkey Tuberculosis AntibodyPositive control.

 

 

1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature  then use, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when

dilute . Washing does not affect the result.

3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .

4. Please make specification curve when you assay, had better make duplicate well, if in the sample the testing material content is excessively high （The sample OD is bigger than the standard well OD 1.5 times ）,please use Sample dilution to dilute certain multiple （n times）,then assay. Please multiply total Dilution Times when calculate（×n×5）.

5.       Closure plate membrane only limits the disposable use, in order to avoid the overlapping pollution

6.       The substrate please evade the light preservation

7.       The test result determination must take the enzyme sign meter reading as a standard

8.       All samples, washing buffer and each kind of reject should according to infective material process.

9.       This reagent which different batch number component do not mix .

10.   If it’s different form English instruction, take English instruction as the standard.

 

 

1．Storage：  2-8℃.

2．validity： six months.