激情综合啪啪6月丁香,久久久久国产精品91福利,99精品日韩欧美在线观看,91成人午夜福利在线观看国产

南京生物試劑網(wǎng)

當前位置:首頁   >>   資料下載   >>   小鼠β淀粉樣蛋白1-40(Aβ1-40)elisa試劑盒說明書英文版

小鼠β淀粉樣蛋白1-40(Aβ1-40)elisa試劑盒說明書英文版

時間:2013/5/23閱讀:289
分享:
  • 提供商

    南京生物試劑網(wǎng)
  • 資料大小

    71.5KB
  • 資料圖片

    查看
  • 下載次數(shù)

    82次
  • 資料類型

    WORD 文檔
  • 瀏覽次數(shù)

    289次
點擊免費下載該資料

Mouse amyloid beta peptide 1-40 (Aβ1-40) ELISA Kit 

 

FOR LABORATORY RESEARCH USE ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

INTENDED USE
This AΒ1-40 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AΒ1-40 in the sample, this AΒ1-40 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AΒ1-40 concentration. The concentration of AΒ1-40 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PRINCIPLE OF THE ASSAY
The coated well immunoenzymatic assay for the quantitative measurement of serum AΒ1-40 utilizes a monoclonal anti- AΒ1-40 as capture antibody and a AΒ1-40-biotin conjugate as detection antibody. The assay sample and standard are incubated together with anti- AΒ1-40antibody coated plate for one hour and aspirated. The diluted AΒ1-40-biotin conjugate is then added to each well and incubated. After the incubation period, the wells are decanted and washed three times. The Streptavidin-HRP is then added to each well and incubated. After the incubation period, the wells are decanted and washed three times. The wells
are then incubated with a substrate for the enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stopping solution is added to stop the reaction, which will then turn the solution yellow.The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is proportional to the AΒ1-40 concentration since AΒ1-40 from samples and AΒ1-40-biotin conjugate compete for the anti- AΒ1-40 antibody binding site. Since the number of sites is limited, as more sites are occupied by AΒ1-40 from the sample, fewer sites are left to bind AΒ1-40-biotin conjugate. Standards of known AΒ1-40concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AΒ1-40. The unknown AΒ1-40 concentration in each sample is interpolated from this curve.

REAGENTS PROVIDED
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. BIOTIN CONJUGATE 100 uL 1 tube, Dilution Buffer 10ml 1 vial
3. STANDARD 800 pg/mL 1 tube, Dilution Buffer 15ml 1 vial
4. STREPTAVIDIN-HRP 100 uL 1 tube, Dilution Buffer 10ml 1 vial
5. SUBSTRATE A 6.0 mL 1 vial
6. SUBSTRATE B 6.0 mL 1 vial
7. STOP SOLUTION 6.0 mL 1 vial
8. WASH SOLUTION x30 10 mL 1 vial
9. Instruction 1

SAMPLE COLLECTION AND STORAGE
Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximay 1000 x g.Remove serum and assay immediay or aliquot and store samples at -20 °C or -80°C.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediay or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.
DO NOT USE HEAT-TREATED SPECIMENS.

MATERIALS REQUIRED BUT NOT SUPPLIED
1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 ml to 1 ml volumes. .
3. Adjustable 10ml -100ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.)
6. Absorbent paper.
7. 37°C incubator.
8. Distilled or deionized water.
9. Data analysis and graphing software..
10. Tubes to prepare standard or sample dilutions.

PRECAUTIONS
1. Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.
3. Do not use kit components beyond their expiration date.
4. Use only deionized or distilled water to dilute reagents.
5. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
6. Use fresh disposable pipette tips for each transfer to avoid contamination.
7. Do not mix acid and sodium hypochlorite solutions.
8. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from human blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed.
9. All samples should be disposed of in a manner that will inactivate viruses.
10. Solid Waste: Autoclave 60 min. at 121°C.
11. Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should be allowed to stand for a minimum of 30 minutes to inactivate the viruses before disposal.
12. Substrate Solution is easily contaminated. If bluish prior to use, do not use.
13. Substrate B contains 20% acetone, keep this reagent away from sources of heat or flame.
14. Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C).
SAMPLE PREPARATION
Serum and plasma samples require a dilution into Standard Dilution Buffer. A
suggested 2-fold dilution is 75 μL sample + 75 μL Standard Dilution Buffer. Mix well.

REAGENT PREPARATION
Standard -The Kit provides a stock solution of 800 pg/mL standard. Allow the standard to sit for a minimum of 5 minutes with gentle mixing prior to making dilutions.Pipette 250μL of Standard Dilution into each tube.
(total 6 tubes) Use the 250μL of stock solution to produce a 2-fold dilution series (including 800 pg/mL,400 pg/mL,200 pg/mL,100 pg/mL,50 pg/mL,25 pg/mL,0 pg/mL). Mix each tube thoroughly before the next transfer. The undiluted Mouse AΒ1-40 Standard serves as the high standard (800 pg/mL). Standard Dilution serves as the zero standard (0 pg/mL).
Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have compley dissolved. To prepare enough Wash Buffer for one plate, add 20mL Wash Buffer Concentrate into deionized or distilled water to prepare 600mL of Wash Buffer.

ASSAY PROCEDURE
Prepare all Standards before starting assay procedure (see Preparation Reagents). It is recommended that all Standards and Samples be added in duplicate to the Microtiter Plate.
1. secure the desired number of coated wells in the holder, then add 100 μL of Standards or Samples to the appropriate well of the antibody pre-coated Microtiter Plate. Cover and incubate for 1 hours at 37°C. Wash the Microtiter Plate using one of the specified methods indicated below: Manual Washing: Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well compley with distilled or de-ionized water, then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure four more times for a total of THREE washes. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate frame firmly hen washing the plate to assure that all strips remain securely in frame. Automated Washing: Aspirate all wells, then wash plate THREE times using distilled or de-ionized water. Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350 μL/well/wash (range: 350-400 μL). After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. It is recommended that the washer be set for a soaking time of 10 seconds or shaking time of 5 seconds between washes
2. Diluted biotin Conjugate to 10ml by Dilution Buffer, Add 100 μL of Diluted Conjugate to each well. Mix well. Complete mixing in this step is important. Cover and incubate for 1 hours at 37°C. Wash the Microtiter Plate using one of the specified methods indicated in 1.

3. Diluted Streptavidin-HRP to 10ml by Dilution Buffer, Add 100 μL of Working Dilution to each well. Cover the plate and incubate for 20 minutes at room temperature.Avoid placing the plate in direct light. Wash the Microtiter Plate using one of the specified methods indicated in 1.
4. Prepare Substrate Solution no more than 15 minutes before end of incubation (see Preparation of Reagents). Add 50 μL Substrate A&B to each well. Cover and incubate for 15 minutes at 20-25°C.
5. Add 50 μL of Stop Solution to each well. Mix well.
6. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 30 minutes.

會員登錄

×

請輸入賬號

請輸入密碼

=

請輸驗證碼

收藏該商鋪

X
該信息已收藏!
標簽:
保存成功

(空格分隔,最多3個,單個標簽最多10個字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時間回復您~

以上信息由企業(yè)自行提供,信息內(nèi)容的真實性、準確性和合法性由相關(guān)企業(yè)負責,環(huán)保在線對此不承擔任何保證責任。

溫馨提示:為規(guī)避購買風險,建議您在購買產(chǎn)品前務(wù)必確認供應(yīng)商資質(zhì)及產(chǎn)品質(zhì)量。

在線留言
国产精品二区高清在线-91精品91久久久久久| 久久免费观看归女高潮特黄-黄色av一本二本在线观看| 亚洲产国偷v产偷v自拍性色av-亚洲欧美日韩国产三区| 欧美成人精品巨臀大屁股-亚洲综合欧美日韩一区| 亚洲av专区在线观看国产-丰满人妻av一区二区三区| 免费av一区在线观看-国产精品视频高潮流白浆视频免费| 亚洲精品一区网站在线观看-黄页视频免费观看网站| 色婷婷六月婷婷一区二区-91草草国产欧美在线观看| 日本韩国亚洲欧美三级-日本东京不卡网一区二区三区| 国产av剧情护士麻豆-三级国产精品欧美在线观看| 久色高清精品在线国产-国产精品视频一区三区四区| 97人妻精品一区二区三区爱与-日韩精品亚洲专区在线观看| 熟妇勾子乱一区二区三区-欧美爱爱视频一区二区| 在线观看中午中文乱码-2021国产一级在线观看| 欧美字幕一区二区三区-好吊妞欧美一区二区在线观看| 欧美精品午夜一二三区-a屁视频一区二区三区四区| 色综合色综合久久综合频道-埃及艳后黄版在线观看| 久久久噜噜噜久久狠狠50岁-精品一区二区三区av| 韩漫一区二区在线观看-精品国产免费未成女一区二区三区| 人妻互换精品一区二区-夜夜爽一区二区三区视频| 免费看黄色污污的网站-欧美一区二区三区爽爽| 国产传媒中文字幕在线观看-午夜福利视频在线播放观看| 亚洲福利视频免费观看-中文字幕日本不卡一区二区| 男人的天堂久久精品激情-最新亚洲精品a国产播放| 欧美日韩黑人在线播放-51在线精品免费视频观看| 女主播啪啪大秀免费观看-精品99午夜福利影院| 青青草原免费国产在线视频-精品人妻乱码一区二区三区四区| 亚洲精品蜜桃在线观看-国产欧美日韩在线观看精品观看| 四虎成人在线免费视频-亚洲熟女中文字幕天堂| 国语自产偷拍精品视频偷拍-国产伊人这里只有精品视频| 欧美日本亚一级二级三区久久精品-日韩欧美一区二区久久婷婷| 哦啊好大用力欧美视频-麻豆国产传媒片在线观看| 四虎成人在线免费视频-亚洲熟女中文字幕天堂| 亚洲欧洲成视频免费观看-国产福利一区二区在线观看| 欧美日韩精品人妻在线-在线播放中文字幕一区| 在线免费观看黄片喷水-国产精品白丝网站在线观看| 亚洲国产精品一区二区av-日本一级黄色一区二区| 亚洲愉拍自拍欧美精品app-亚洲一区不卡在线视频| av中文字幕男人天堂-懂色av一区二区三区在线观看| 久久精品国产亚洲av湖南-竹菊精品一区二区三区| 在线免费观看黄片喷水-国产精品白丝网站在线观看|