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CRL-2713 MDA-kb2 人乳腺細(xì)胞

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CRL-2713 MDA-kb2 人乳腺細(xì)胞 的詳細(xì)介紹

CRL-2713 MDA-kb2 人乳腺細(xì)胞

ATCC^® Number:

CRL-2713™

Price:

Designations:	MDA-kb2
Depositors:	VS Wilson
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: mammary gland; breast Cell Type: epithelial
Cellular Products:	luciferase （ [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors （GR） and androgen receptors （AR）.）
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: November 25, 1998
Applications:	assay of androgen receptor （ [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.）
Receptors:	androgen receptor [57526] glucocorticoid receptor, positive [57526]
DNA Profile （STR）:	Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 11 D7S820: 10 THO1: 6 TPOX: 10 vWA: 17,18
Age:	48 years adult
Gender:	female
Ethnicity:	Caucasian
Comments:	Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, * Temperature: 37.0°C
Subculturing:	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. *Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008 recommended serum:ATCC 30-2020 parental cell line:ATCC HTB-131
References:	57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974

CRL-2713 MDA-kb2 人乳腺細(xì)胞的詳細(xì)介紹

CRL-2713 MDA-kb2 人乳腺細(xì)胞

ATCC^® Number:

CRL-2713™

Price:

Designations:	MDA-kb2
Depositors:	VS Wilson
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	adherent
Organism:	Homo sapiens （human）
Morphology:	epithelial
Source:	Organ: mammary gland; breast Cell Type: epithelial
Cellular Products:	luciferase （ [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors （GR） and androgen receptors （AR）.）
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation:	Isolation date: November 25, 1998
Applications:	assay of androgen receptor （ [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.）
Receptors:	androgen receptor [57526] glucocorticoid receptor, positive [57526]
DNA Profile （STR）:	Amelogenin: X CSF1PO: 10,12 D13S317: 12 D16S539: 9 D5S818: 11 D7S820: 10 THO1: 6 TPOX: 10 vWA: 17,18
Age:	48 years adult
Gender:	female
Ethnicity:	Caucasian
Comments:	Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, * Temperature: 37.0°C
Subculturing:	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. *Subction Ratio:** A subction ratio of 1:2 to 1:4 is recommended Medium Renewal:* Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium supplemented with 5% （v/v） DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2008 recommended serum:ATCC 30-2020 parental cell line:ATCC HTB-131
References:	57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974

的詳細(xì)介紹

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人胰腺癌細(xì)胞 Panc 08.13 CRL-2551
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人骨髓瘤細(xì)胞 U266 TIB-196
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人多形性惡性膠質(zhì)瘤 T98G [T98-G] CRL-1690
人神經(jīng)母細(xì)胞瘤細(xì)胞 BE（2）-C CRL-2268
人腎透明細(xì)胞癌細(xì)胞 Caki-2 HTB-47
人皮膚成纖維細(xì)胞 Hs 865.Sk CRL-7601

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HTB-113 HEC-1-B 人子宮內(nèi)膜腺癌細(xì)胞
AN3 CA 人子宮內(nèi)膜腺癌細(xì)胞
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HTB-58 SK-MES-1 人肺鱗癌細(xì)胞
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HTB-79 Capan-2 人胰腺癌細(xì)胞
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CRL-2922 Eayh926 人臍靜脈細(xì)胞融合細(xì)胞
WB-F344 大鼠肝干細(xì)胞
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RSC96大鼠雪旺細(xì)胞
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