目的評(píng)價(jià)3種人體片形吸蟲(chóng)病ELISA試劑盒的檢測(cè)效果。方法采用本研究室大片吸蟲(chóng)抗原、肝片吸蟲(chóng)成蟲(chóng)可溶性抗原包被的人體片形吸蟲(chóng)病ELISA試劑盒(Fg ELISA和Fh ELISA)及德國(guó)DRG公司生產(chǎn)的人體片形吸蟲(chóng)IgG抗體ELISA試劑盒(DRG ELISA),分別檢測(cè)26份大片吸蟲(chóng)患者血清、180份其他寄生蟲(chóng)病患者血清和26份健康人血清,評(píng)價(jià)3種檢測(cè)試劑盒的檢測(cè)效果。結(jié)果Fg ELISA、Fh ELISA和DRG ELISA 3種檢測(cè)盒的敏感性分別為100.0%、80.8%(95%CI:65.7%～95.9%)和100.0%,特異性分別為87.9%(95%CI:83.5%～92.4%)、85.0%(95%CI:80.1%～89.9%)和83.5%(95%CI:78.4%～88.6%),約登指數(shù)分別為0.88、0.66和0.84。其中,Fg ELISA檢出率(100%)明顯高于Fh ELISA(80.8%)(P<0.05);Fg ELISA檢測(cè)26例大片吸蟲(chóng)病患者血清的吸光度(A)值(A/CO)為1.70,明顯高于Fh ELISA的1.18(P<0.000 1)。結(jié)論Fg ELISA試劑盒檢測(cè)效果較好,且成本相對(duì)較低,比Fh ELISA和DRG ELISA兩法更適宜用于我國(guó)西南大片吸蟲(chóng)病流行區(qū)臨床樣本檢測(cè)及大規(guī)模篩查。

3ELISACVFgELISAFhELISADRGELISA3CV8.17%9.29%5.69%15%A/CODRGELISAFgELISAFhELISAFgELISAFhELISADRGELISA3100.0%80.8%100.0%87.9%85.0%83.5%1318FgELISA100%FhELISA80.8%P<0.0/CO1.70FhELISA1.18P<0.0001FgELISAFhELISA351.0%40.4%43.3%100.0%97.2%100.0%3FgELISAFhELISADRGELISA333.3%10/3040.0%12/3036.7%11/3075.0%12/1681.3%13/1681.3%13/163IndexNo.truepositivesNo.falsepositivesNo.truenegativesNo.falsenegativesSensitivity%95%CISpecificity%95%CIPositivepredictivevalue%95%CINegativepredictivevalue%95%/COTable4A/COvaluecomparisonof3ELISAkitsindetectionofserumsamplesfrompatientswithfasciolasis··180