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產(chǎn)品名稱:磷酸化自噬相關(guān)蛋白4C抗體,phgophs-ATG4C(Ser451)現(xiàn)貨
產(chǎn)品編號:BYK-5196R
產(chǎn)品規(guī)格: 0.1ml/0.2ml
抗體來源:Rabbit OR Mouse
產(chǎn)品用途:科研實(shí)驗(yàn),用于免疫組化實(shí)驗(yàn),WB、IF、elisa實(shí)驗(yàn)
性 狀: Lyophilized or Liquid
濃 度: 1mg/1ml
亞 型: IgG
貯 存: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications..
相關(guān)標(biāo)記: HRP Biotin Gold RBITC AP FITC Cy3 Cy5 Cy5.5 Cy7 PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 APC Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647
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磷酸化自噬相關(guān)蛋白4C抗體,phgophs-ATG4C(Ser451)現(xiàn)貨免疫組化:
石蠟包埋組織切片3~4μm 厚度
1.烤片: 將待做切片置于切片架上,于60℃恒溫烤箱中至少烤1 hr;
2.脫蠟: 切片放入盛有二甲苯的容器中脫蠟3次(即二甲苯Ⅰ、Ⅱ、Ⅲ),每次10 min;
3.水化: 切片經(jīng)下行酒精水化,無水乙醇5min,95%乙醇2次(每次2min),85%乙醇2 min;75%乙醇2min,自來水沖洗,ddH2O洗2×2min;
4.抗原修復(fù): 根據(jù)抗體說明書*方法進(jìn)行抗原修復(fù),常采用高壓、微波(溫度達(dá)到98~100℃)或酶消化修復(fù)法,室溫自然冷卻,自來水沖洗,ddH2O洗2×2min,TBS洗滌(2×2min)(具體修復(fù)方法見附1)* 注:有些抗原勿需修復(fù),直接進(jìn)入第5步封閉。
5.封閉: 滴加試劑B,37℃濕盒孵育30 min;
6.加一抗:滴加用試劑C(選擇合理的工作比例),37℃濕盒孵育2 hr 或4℃過夜;
7.洗滌: TBS-T洗滌(3×5 min);
8.封閉: 滴加試劑B,37℃濕盒孵育10 min;
9.加二抗: 滴加用抗體稀釋液稀釋的*化二抗(試劑D),37℃濕盒中孵育30 min;
10.洗滌: TBS-T洗滌(3×5 min);
11.封閉: 滴加試劑Tween 20,37℃濕盒孵育封閉20 min;
12.加HRP-SA: 滴加用試劑C稀釋的試劑E(1:50~200,終濃度5~20 nM),37℃濕盒中孵育30 min;
13.洗滌: TBS-T洗滌(3×5 min),TBS洗滌(2×5 min);
14.顯色:應(yīng)用DAB溶液(試劑F)顯色;
15.復(fù)染:自來水充分沖洗,復(fù)染,脫水,透明;
16.封片: 待組織標(biāo)本干后,用試劑緩沖甘油封固劑封片;
17.觀察成像: 顯微鏡下觀察成像。
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