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Mouse Anti-MIP-1a

閱讀:172發(fā)布時間:2012-8-21

 

                Mouse Anti-MIP-1a
Catalog Number : RS-2452R    
Quantity size :   0.2ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )
 
Background:     Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximay 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.                     
 
Specificity :        · anti- MIP-1a is a mouse monoclonal antibody unconjugated
 · specific for MIP-1a of human and rat
· epitope mapping near the N-terminus of MIP-1a of human
       and rat origin
·use for western blotting,elisa,immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 20kDa
                      Mouse Anti-MIP-1a           
 
Application:       · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:     Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.

                Mouse Anti-MIP-1a


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