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專業(yè)試劑批發(fā)銷售>技術(shù)文章>Rabbit Anti-CD25抗體說明書
技術(shù)文章

Rabbit Anti-CD25抗體說明書

閱讀：256發(fā)布時(shí)間：2013-4-14

Rabbit Anti-CD25抗體說明書

Cat. Number:  Rs-0577R

 

Quantity size:  0.2ml  （dilute with pH 7.4    0.01 M PBS or diluent of antibody）

 

Background: CD25（IL2） receptor alpha （IL2RA） and beta （IL2RB） chains, together with the common gamma chain （IL2RG）, constitute the high-affinity IL2 receptor. Homodimeric alpha chains（IL2RA） result in low-affinity receptor, while homodimeric beta （IL2RB） chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternay-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.

 

Specificity:       ·anti- CD25 is a rabbit monoclonal antibody unconjugated

                ·specific for CD25 of rat  

                ·epitope mapping near the amino terminus of CD25 of  

                   rat origin

                · use for western blotting, elisa, immunoprecipitation    

                   and immunohistochemistry

·  Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 82kDa

 

Application:     · Western blotting: 1:100-500

· Immunohistochemistry: 1:100-500

· ELISA: 1:500-1000

·  Optimal working dilutions must be determined by the end user.

 

Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note:  This product as supplied is  intended for research use only, not for use in human, therapeutic or diagnostic applications.

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