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專業(yè)試劑批發(fā)銷售>技術(shù)文章>Rabbit Anti- ALDH1A1抗體說(shuō)明書
技術(shù)文章

Rabbit Anti- ALDH1A1抗體說(shuō)明書

閱讀：368發(fā)布時(shí)間：2013-4-12

Rabbit  Anti- ALDH1A1抗體說(shuō)明書

FROM：Abcam

Catalog Number : ab52492

Quantity size :   100ul  （dilute with pH 7.4  0.01 M PBS or antibody diluent ）

 

Background:

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.                      

Specificity :        · anti- ALDH1A1 is a Rabbit monoclonal antibody unconjugated

 · specific for ALDH1A1 of Mouse, Rat, Human

·use for western blotting,elisa, immunoprecipitation   and immunohistochemistry

·  Protein G affinity chromatography purification, purity :>95%

· Isotype: IgG

·  mol wt: 55kDa

                

Application:       · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

·  Optimal working dilutions must be determined by the end user.

 

Storage:     Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

 

Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.


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