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>技術文章>Mouse Anti-Integrin αV+β3/CD51+CD61
技術文章

Mouse Anti-Integrin αV+β3/CD51+CD61

閱讀:150發(fā)布時間:2012-8-21

 

Mouse Anti-Integrin αV+β3/CD51+CD61  
From:   Santa Cruz
 
Cat. Number:  Rs-1310R  
 
Quantity size:  0.2ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background:    Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells. ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
 
Mouse Anti-Integrin αV+β3/CD51+CD61  
Specificity:      · anti- Integrin αVβ3 is a mouse monoclonal antibody unconjugated
                · specific for Integrin αVβ3 protein of human, mouse, rat, pig and rabbit    
                · epitope mapping near the amino terminus of Integrin αVβ3 protein of
human, mouse, rat, pig and rabbit
                · use for elisa, immunoprecipitation and immunohistochemistry  
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG   
 
Application:     ·Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Mouse Anti-Integrin αV+β3/CD51+CD61  


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