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Rabbit Anti-CD74/MHC II

閱讀:261發(fā)布時(shí)間:2012-8-6

 

                 Rabbit  Anti-CD74/MHC II
 
Catalog Number :  RS-2518R
Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )
Background:   :CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.
 
Specificity :  · anti-Anti- MHC II is a rabbit monoclonal antibody unconjugated
· use for western blotting, elisa, immunoprecipitation and immunohistochemistry
· Protein G affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 60 kDa
               
Cross reactivity: Reacts with Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Fruit fly (Drosophila melanogaster), Monkey
 
Rabbit  Anti-CD74/MHC II
Application:  ·Western blotting 1:100-500
             ·Immunohistochemistry 1:100-500
             ·ELISA 1:500-1000
             ·Optimal working dilutions must be determined by the end user.
Storage:    Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 

Rabbit  Anti-CD74/MHC II


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