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當(dāng)前位置：上海力敏實(shí)業(yè)有限公司>>生化試劑、抗體、血清>>常規(guī)生化試劑>> Qiagen凱杰981103 Strip Tubes and Caps 0.1ml
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更新時(shí)間：2023-07-26 17:59:06瀏覽次數(shù)：2404次
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何先生
sigma現(xiàn)貨代理Dispase II D-4693
美天旎miltenyi磁珠緩沖液MACS Running Buffer
invivogen支原體清除試劑Plasmocin treatment
BIO-RAD伯樂ECL化學(xué)發(fā)光底物Clarity Western E
takara RR420A SYBR Premix Ex Taq*
Qiagen凱杰981103 Strip Tubes and Caps 0.1ml
操作步驟：應(yīng)用QIAGEN Plasmid Kits純化過程中，通過離心取出細(xì)菌裂解液。然后將澄清的裂解液裝入陰離子交換柱，在適當(dāng)?shù)牡望}濃度和pH值條件下，陰離子交換柱會(huì)選擇性地結(jié)合質(zhì)粒DNA。RNA、蛋白質(zhì)、代謝物和其他小分子雜質(zhì)通過中鹽濃度洗滌去除，應(yīng)用高鹽濃度緩沖夜洗脫超純的質(zhì)粒DNA（見流程圖"Comparison of QIAGEN Plasmid Kit Procedures"）。通過異丙醇沉淀濃縮并脫鹽DNA，離心法收集DNA。
Qiagen凱杰981103 Strip Tubes and Caps 0.1ml，
IAGEN Plasmid Kits 純化所得的質(zhì)粒DNA非常適合于諸如轉(zhuǎn)染（見圖"轉(zhuǎn)染效率 Vs. 質(zhì)粒純化方法"）、克隆、手動(dòng)或自動(dòng)測(cè)序（包括毛細(xì)管測(cè)序）和體外轉(zhuǎn)錄等應(yīng)用。
純化PCR產(chǎn)物，100bp-10kb
高達(dá)95%的回收效率
三步驟回收DNA，片段zui大可達(dá)10 kb
上樣液含有三色膠示蹤染料，方便樣本分析
Cat No./ID: 9018895 Rotor-Disc 100 Rotor Log in For holding Rotor-Disc 100 discs with reaction volumes of 15–25 µl in the Rotor-Gene Q; requires Rotor-Disc 100 Locking Ring |
Cat No./ID: 9018896 Rotor-Disc 100 Locking Ring Log in For locking a Rotor-Disc 100 in the Rotor-Disc 100 Rotor |
Cat No./ID: 9018897 Rotor-Disc Pipetting Aid Log in Aid for marking well during manual reaction setup on a Rotor-Disc Loading Block |
Cat No./ID: 9018898 Rotor-Disc Heat Sealer 110V Log in Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block |
Cat No./ID: 9018899 Rotor-Disc 72 Rotor Log in For holding Rotor-Disc 72 discs with reaction volumes of 20–25 µl in the Rotor-Gene Q; requires Rotor-Disc 72 Locking Ring |
Cat No./ID: 9018900 Rotor-Disc 72 Locking Ring Log in For locking a Rotor-Disc 72 in the Rotor-Disc 72 Rotor |
Cat No./ID: 9018901 Loading Block 72 x 0.1 ml Tubes Log in Aluminum block for manual reaction setup with a single-channel pipet in 72 x 0.1 ml tubes |
Cat No./ID: 9018902 Loading Block 72 x 0.1 ml Multi-channel Log in Aluminum block for reaction set-up with multichannel pipets in 72 x 0.1 ml tubes |
Cat No./ID: 9018903 72-Well Rotor Log in For holding Strip Tubes and Caps, 0.1 ml, with reaction volumes of 10–50 µl; requires Locking Ring 72-Well Rotor |
Cat No./ID: 9018904 Locking Ring 72-Well Rotor Log in For locking Strip Tubes and Caps, 0.1 ml in the 72-Well Rotor |
Cat No./ID: 9018905 Loading Block 96 x 0.2 ml Tubes Log in Aluminum block for manual reaction set-up in a standard 8 x 12 array using 96 x 0.2 ml tubes |
Cat No./ID: 9018906 36-Well Rotor Locking Ring Log in For locking PCR Tubes, 0.2 ml, in the 36-Well Rotor |
Cat No./ID: 9018907 36-Well Rotor Log in For holding PCR Tubes, 0.2 ml, with reaction volumes of 20–50 µl; requires 36-Well Rotor Locking Ring |
Cat No./ID: 9018908 Rotor Holder Log in Metal free-standing holder for assembling tubes and Rotor-Discs into rotors |
Cat No./ID: 9018910 Rotor-Disc 72 Loading Block Log in Aluminium block for manual and automated reaction setup in Rotor-Disc 72 discs |
Cat No./ID: 9019725 Rotor-Disc Heat Sealer 230V Log in Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block |
Cat No./ID: 9020286 Loading Block, Rotor-Disc 100 Easylift Ejector Inquire Ejector to be used in combination with Loading Block, Rotor-Disc 100 Easylift (cat. no. 9020287) |
Cat No./ID: 9020287 Loading Block, Rotor-Disc 100 Easylift Inquire Aluminium block for manual and automated reaction setup in Rotor-Disc 100 discs. The block covers 2 standard SBS positions in a vertical orientation on the QIAgility instrument worktable; ejection of the Rotor-Disc 100 is made convenient by using the Easylift ejector (cat. no. 9020286) |
Cat No./ID: 981005 PCR Tubes, 0.2 ml (1000) Log in 1000 thin-walled tubes for 1000 reactions of 20–50 µl |
Cat No./ID: 981008 PCR Tubes, 0.2 ml (10000) Log in 10 x 1000 thin-walled tubes for 10,000 reactions of 20–50 µl |
Cat No./ID: 981103 Strip Tubes and Caps, 0.1 ml (250) Log in 250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl |
Cat No./ID: 981106 Strip Tubes and Caps, 0.1 ml (2500) Log in 10 x 250 strips of 4 tubes and caps for 10,000 reactions of 10–50 µl |
Cat No./ID: 981301 Rotor-Disc 72 (24) Log in 24 individually wrapped discs for 1728 reactions of 20–25 µl |
Cat No./ID: 981303 Rotor-Disc 72 (240) Log in 10 x 24 individually wrapped discs for 17,280 reactions of 20–25 µl |
Cat No./ID: 981311 Rotor-Disc 100 (30) Log in 30 individually wrapped discs for 3000 reactions of 15–25 µl |
Cat No./ID: 981313 Rotor-Disc 100 (300) Log in 10 x 30 individually wrapped discs for 30,000 reactions of 15–25 µl |
Cat No./ID: 981601 Rotor-Disc Heat Sealing Film (60) Log in 60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs |
Cat No./ID: 981604 Rotor-Disc Heat Sealing Film (600) Log in 10 x 60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs |
Rotor-Gene Q Accessories are for use with the Rotor-Gene Q thermal cycler.
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