肌細(xì)胞培養(yǎng)基是專門為正常人類心肌細(xì)胞體外培養(yǎng)設(shè)計(jì)的zui適于其生長(zhǎng)的培養(yǎng)基，是經(jīng)滅菌的液體培養(yǎng)基。包含必需和非必需氨基酸、維生素、有機(jī)和無(wú)機(jī)化合物、激素、生長(zhǎng)因子、微量礦物質(zhì)和低濃度胎牛血清(5%)。該培養(yǎng)基緩沖體系為重碳酸鹽，在含5%CO2的細(xì)胞培養(yǎng)箱中平衡后pH值為7.4。該培養(yǎng)基的配方能夠選擇性的促進(jìn)正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)中的增殖和生長(zhǎng)，并為其達(dá)到營(yíng)養(yǎng)平衡狀態(tài)。

心肌細(xì)胞培養(yǎng)基包含500 ml基礎(chǔ)培養(yǎng)基，25ml胎牛血清 (FBS, Cat. No. 0025),5ml心肌細(xì)胞生長(zhǎng)添加物 (CMGS, Cat. No. 6252) 和5 ml青霉素/鏈霉素溶液 (P/S, Cat. No. 0503).

產(chǎn)品使用說(shuō)明：僅供科研研究使用

參考文獻(xiàn)：

1.) Cai, W., Yang, X., Han, S., Guo, H., Zheng, Z., Wang, H., Guan, H., Jia, Y., Gao, J., Yang, T., Zhu, X. & Hu, D. (2016) Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2/STAT3 Signaling Oxid Med Cell Longev. 2016

2.) Davy, P.M., Lye, K.D., Mathews, J., Owens, J.B., Chow, A.Y., Wong, L., Moisyadi, S. & Allsopp, R.C. (2015) Human adipose stem cell and ASC-derived cardiac progenitor cellular therapy improves outcomes in a murine model of myocardial infarction Stem Cells Cloning. 8

3.) d'Avenia, M., Citro, R., De Marco, M., Veronese, A., Rosati, A., Visone, R., Leptidis, S., Philippen, L., Vitale, G., Cavallo, A., Silverio, A., Prota, C., Gravina, P., De Cola, A., Carletti, E., Coppola, G., Gallo, S., Provenza, G., Bossone, E., Piscione, F., Hahne, M., De Windt, L.J., Turco, M.C. & De Laurenzi, V. (2015) A novel miR-371a-5p-mediated pathway, leading to BAG3 upregulation in cardiomyocytes in response to epinephrine, is lost in Takotsubo cardiomyopathy Cell Death Dis. 6

4.) Khurana S, Hollingsworth A, Piche M, Venkataraman K, Kumar A, Ross GM, Tai TC. (2014) "Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H 2 O 2." Oxid med cell longev. 657512.

5.) Wang E, Sun S, Qiao B, Duan W, Huang G, An Y, Xu S, Zheng Y, Su Z, Gu X, Jin L, Wang H. (2013) "Identification of functional mutations in GATA4 in patients with congenital heart disease." PLoS One. 8: e62138.

6.) Jian B, Wang D, Chen D, Voss J, Chaudry I, Raju R. (2010) "Hypoxia-induced alteration of mitochondrial genes in cardiomyocytes: role of Bnip3 and Pdk1." Shock. 34: 169-75.