牛LIF ELISA試劑盒 說(shuō)明書(shū):
ELISA 試驗(yàn)時(shí),注意事項(xiàng)如下:
正式試驗(yàn)時(shí),應(yīng)分別以陽(yáng)性對(duì)照與陰性對(duì)照控制試驗(yàn)條件,待檢樣品應(yīng)作一式二份,以保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性。有時(shí)本底較高,說(shuō)明有非特異性反應(yīng),可采用羊血清、兔血清或BSA等封閉。
Elisa試劑盒實(shí)驗(yàn)規(guī)則:
1、溫浴時(shí),要用不干膠或膠帶紙封好酶標(biāo)板,防止水分的蒸發(fā)。
2、洗板時(shí),每次洗液加入后,應(yīng)靜置1分鐘,使清洗更加*。沒(méi)有洗板機(jī)時(shí),倒去液體后,要將酶標(biāo)板在報(bào)紙或毛紙上用力拍干。
3、洗液不夠時(shí),可用蒸餾水自行配制PH7.4,0.02M的磷酸緩沖液,加入0.1%的吐溫20作為洗液。加入1/1000的疊氮鈉后可*保存。
4、底物是光敏感的,要在臨用前現(xiàn)配。
5、檢測(cè)前,要打開(kāi)酶標(biāo)儀,使之穩(wěn)定10分鐘以上。
6、底物有一定的毒性,終止液對(duì)皮膚有腐蝕性,應(yīng)盡量避免接觸。
7、待檢樣品要澄清,否則會(huì)影響結(jié)果。
8、溫浴時(shí)間應(yīng)遵守試劑盒規(guī)定。
產(chǎn)品免費(fèi)代測(cè):牛LIF ELISA試劑盒
英文名稱:Bovine Leukemia inhibitory KXctor,LIF ELISA KIT
規(guī)格:96T/48T,定量/定性
保存條件:2-8℃,6個(gè)月
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