產(chǎn)品名稱：BRAIN HEART INFUSION BROTH
貨號(hào)：oxoid CM1135
規(guī)格：500g
產(chǎn)品說(shuō)明：
BRAIN HEART INFUSION BROTH
Code: CM1135
Dissolve 37g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.
A versatile liquid infusion medium which is suitable for the c*tion of streptococci, Neisseria and other fastidious organisms, this medium is recommended for blood culture work and, with the additions described below, for the isolation and c*tion of pathogenic fungi.
Oxoid Brain Heart Infusion Broth is essentially a buffered infusion broth, giving similar results to the brain dextrose broths originally employed for the c*tion of streptococci1, and for the c*tion of dental pathogens2. The addition of 0.1% agar will serve to reduce convection currents and so create conditions of varying oxygen tension which favour the growth and primary isolation of aerobes and anaerobes3, while even easily c*ted organisms show improved growth4.
Oxoid Brain Heart Infusion Broth was used in a test for the pathogenicity of streptococci5,6 and the same medium was enriched with ascitic fluid for the c*tion of gonococci7. It is especially useful as a growth and suspension medium for staphylococci which are to be tested for coagulase production; Newman8 employed a similar medium for this purpose in an investigation of food poisoning caused by dairy products.
A satisfactory medium for blood culture can be prepared by adding 1g of agar per litre of Brain Heart Infusion Broth. Ensure that the agar is uniformly distributed in the sterile broth before dispensing into bottles. More conveniently, add one Agar Tablet （CM0049） to each 100ml of Brain Heart Infusion and sterilize by autoclaving for 15 minutes at 121°C. Cool to 60-70°C and mix gently to ensure uniform distribution of the agar.
Tubes of Oxoid Brain Heart Infusion Broth which are not used the same day as sterilized should be placed in a boiling water bath for several minutes to remove absorbed oxygen, and cooled rapidly without shaking, just before use.
Further supplements to improve the recovery of organisms from blood can be added before sterilization or aseptically post-sterilization. Co-enzyme1 （NAD）, penicillinase and p-amino benzoic acid are examples. Brain Heart Infusion Broth supplemented with yeast extract, haemin and menadione was consistently better in producing heavy growth of five species of Bacteroides than three standard anaerobic broths. Furthermore, microscopy of overnight cultures showed normal morphology in Brain Heart Infusion Broth but abnormal morphology in the three anaerobic broths9.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store tubed or bottled medium in the dark and below 20°C.