Description
Analysis Note Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol Actions Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation Note Highly purified by specific affinity chromatography. Purified by modification of the method of Matsumoto.1
Properties
type Type VI
form lyophilized powder
potency <20 μg per mL agglutination activity
total impurities Carbohydrate, essentially free
salt, essentially free
solubility PBS: 4 % （w/v）
storage temp. −20°C
Safety
Personal Protective Equipment dust mask type N95 （US）, Eyeshields, Faceshields, Gloves
Hazard Codes Xn
Risk Statements 42/43
Safety Statements 24/25-36
WGK Germany 3
RTECS GK6890000
References
Cited Reference 1. Matsumoto, et al. Anal. Biochem. 116, 103, （1981） Abstract
reference Reeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, （1974）
Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, （1968）
Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, （1973）
, Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , （1976）
Merck Merck 13,2521
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