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AequoZen FroZen cells, Muscarinic M1, Human Recombinant, CHO-K1

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Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazin......

詳細(xì)介紹

Frozen Cells validated for calcium testing: Muscarinic M1 receptor, human recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.

Features:

Pre-validated batches of ready-to-use cells for calcium testing
Optimized to alleviate biological variability
Step-by-Step protocols for culture-free functional testing on our validated cells
Affordable access to small aliquots of cells (minimum size: 10 million cells/vial)
Ready-to-use cells for Profiling, Pharmacology or Screening applications
Up-scaling and customized developments

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.

規(guī)格

檢測目標(biāo)類	GPCR
檢測目標(biāo)類型	Cell line
檢測驗(yàn)證	Calcium Luminescence
G-Alpha天然受體	Gq/G11
宿主細(xì)胞	CHO-K1
產(chǎn)品品牌名稱	AequoZen
受體類型	Acetylcholine (Muscarinic), Muscarinic
第二信使釋放	Calcium flux
運(yùn)輸條件	干冰
治療領(lǐng)域	Cardiovascular, Parasympathetic Nervous System, Gastrointestinal
產(chǎn)品尺寸	10 million cells