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ACQUITY UPLC HSS Cyano Column

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不封端的丙基氰基；可被成功用于正相和反相分離，HSS CN是最多樣化的固定相。

詳細介紹

超高性能通用的丙基氰基鍵合相，提供*的峰形，增加保留，在低PH值條件下具有延長柱壽命。高強度硅膠顆粒（HSS）技術(shù)專為補充雜化BEH和CSH顆粒開發(fā)，用于提高色譜效率。高孔徑容量的硅膠基質(zhì)HPLC顆粒不具備必要的機械穩(wěn)定性來承受UPLC分離過程中的壓力，如果實驗室需要將現(xiàn)有的HPLC方法轉(zhuǎn)移到UPLC系統(tǒng)上的話，這一點就顯得尤為重要。機械穩(wěn)定性的限制促使沃特世的科學(xué)家設(shè)計出一種顆粒具有高機械穩(wěn)定性和較長的使用壽命以及高壓下的UPLC的柱效。


與直鏈烷烴色譜柱相比，ACQUITY UPLC HSS氰基色譜柱提供低疏水性和選擇性。由于采用創(chuàng)新的氰基單點鍵合配以保護性烷基側(cè)鏈，ACQUITY UPLC HSS氰色譜柱在低至中等pH條件下具有超穩(wěn)定的保留性能、優(yōu)異峰形和可重現(xiàn)性。另外HSS氰色譜柱同時兼容于反相和正相技術(shù)。


Simultaneous determination of etonogestrel and ethinyl estradiol in human plasma by UPLC-MS/MS and its pharmacokinetic study
A selective, sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of etonogestrel (ENG) and ethinyl estradiol (EE) in human plasma. The analytes and their deuterated internal standards, ENG-d7 and EE-d4, were extracted from plasma samples by solid-phase extraction on HyperSep™ Retain PEP cartridges. The chromatographic analysis was performed on an Acquity UPLC HSS Cyano column, 100 ? (50 × 2.1 mm, 1.8 μm), column using gradient phase, acetonitrile and 2.0 mm ammonium trifluoroacetate at 0-1.7 min (65:35, v/v) and 1.8-2.7 min (95:5, v/v) with 0.250 mL/min flow rate. Analytes and IS protonated precursor → product ion transitions (ENG, m/z 325.2 → 257.2; EE, m/z 530.2 → 171.2; ENG-d7, m/z 332.2 → 263.2; EE-d4, m/z 534.2 → 171.2) were monitored on a Triple Quadrupole Mass spectrometer (TQMS), operating in multiple reaction monitoring and positive ionization mode. The calibration curves were established at 10.00-2500 pg/mL for ENG and 1.500-150.0 pg/mL for EE with a correlation coefficient (r2 ) ≥0.9996 for both. The validated method was successfully applied to support a bioequivalence study of 0.15 mg ENG and EE 0.03 mg tablet formulation, administered in 24 healthy Indian females. Method reliability was assessed by reanalysis of 94 incurred study samples.


品名

編號

ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100?, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 100 - 15...

186006002

ACQUITY - Xselect HSS Cyano (CN) 100?, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2/pkg

186006001

ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100?, 1.8 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm...

186006000

ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg

186005996

ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg

186005997

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 100 mm, 1/pkg

186005993

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 75 mm, 1/pkg

186005992

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 150 mm, 1/pkg

186005994

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 50 mm, 1/pkg

186005991

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 30 mm, 1/pkg

186005990

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 1/pkg

186005989

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 75 mm, 1/pkg

186005987

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 1/pkg

186005988

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 1/pkg

186005985

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 1/pkg

186005986

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg

176002709

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 150 mm, 1/pkg

186005984

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 100 mm, 1/pkg

186005983

ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 50 mm, 1/pkg

186005982

ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg

186005998

ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg

186005999

ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column, 100?, 1.8 µm, 2.1 mm X 5 mm, 3/pkg

186005995


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