Wavelength range FI Standard: Ex 230 – 600 nm, Em 330 – 600 nm Optional Ex 230 – 850 nm, Em 280 – 850 nm FP Standard Ex 300 – 600 nm; Em 330 – 600 nm Optional Em 330 – 850 nm Absorbance 230 – 1000 nm
Detectors Fluorescence PMT, optional UV and red-色nsitive Absorbance UV silicon photodiode Luminescence photon counting system with low dark current PMT
Plate formats 6- to 384-well plates, cuvettes, NanoQuant Plate
Temperature control Ambient +5 °C up to 42 °C
Shaking Linear, orbital
Fluorescence 色nsitivity* Infinite F200 PRO Fluorescence top reading* 85 amol / well (0,85 pM, 384-well plate) Fluorescence bottom reading* 0.7 fmol / well (3,5 pM; 96-well plate) TRF** 2.8 amol / well (28 fM; 384-well plate) FP* < 4 mP standard deviation @ 1nM Fluorescein Infinite M200 PRO Fluorescence top reading* 170 amol / well (1,7 pM; 384-well plate) Fluorescence bottom reading* 1.2 fmol / well (6 pM; 96-well plate) TRF** 90 amol / well (0.9 pM; 384-well plate)
Luminescence 色nsitivity Glow luminescence*** 225 amol ATP / well (9 pM; low volume 384-well plate) Flash luminescence**** 12 amol ATP / well (218 fM; 384-well plate)
Injectors Pump speed 100 – 300 μl/s, 1 μl increments; max. volume 800 μl per stroke Dead volume 100 μl including pump back
Fastest Read Times 96 well plate 20 色c 384 well plate 30 色c Wavelength Ex / Em-scan 150 色c (96 well plate 450 – 550 nm, 5 nm step)
* Detection limit for Flourescein ** Detection limit for Europium *** Detection limit for ATP (144-041 ATP detection kit SL (BioThema) **** Detection for ATP (ENLITER Kit)