人宮頸癌細(xì)胞-HeLa細(xì)胞

人宮頸癌細(xì)胞-HeLa細(xì)胞是一個(gè)來(lái)自人體組織經(jīng)連續(xù)培養(yǎng)獲得的非整倍體上皮樣細(xì)胞系，它由GeyGO等在1951年從31歲女性黑人的宮頸癌組織建立。已知該細(xì)胞系含有人HPV病毒HPV18序列，需在2級(jí)生物安全防護(hù)臺(tái)操作。該細(xì)胞角蛋白陽(yáng)性，p53表達(dá)量較低，但表達(dá)正常水平的pRB（視網(wǎng)膜母細(xì)胞瘤抑制因子）。

細(xì)胞名稱：人宮頸癌細(xì)胞

細(xì)胞簡(jiǎn)稱：HeLa 細(xì)胞

細(xì)胞別稱：HELA; Hela; He La; He-La; Henrietta Lacks cells; Helacyton gartleri

產(chǎn)品貨號(hào)：TCH-C193

規(guī)格：1×10?cells，  T25 培養(yǎng)瓶

種屬來(lái)源：人

組織來(lái)源：宮頸

特征：宮頸癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：MEM+10%FBS+1%P/S

人宮頸癌細(xì)胞-HeLa細(xì)胞 配套培養(yǎng)基貨號(hào)： TCH-G193

傳代比例：1:2-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

實(shí)驗(yàn)室儀器：二氧化碳培養(yǎng)箱

實(shí)驗(yàn)耗材：T25細(xì)胞培養(yǎng)瓶，T75培養(yǎng)瓶,錐形瓶

"American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

AOAC International Invasiveness by Escherichia coli of mammalian cells, microbiological method. Gaithersburg, MD:AOAC International;AOAC ""Official Methods of Analysis of the AOAC International"" 982.36.

Baldi A, et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454

Gey GO, et al. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12: 264-265, 1952.

Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844

Boshart M, et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740

Schneider-Gadicke A, Schwarz E. Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067

Schwarz E, et al. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314: 111-114, 1985. PubMed: 2983228

Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Jones HW Jr., et al. George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin. Obstet. Gynecol. 38: 945-949, 1971. PubMed: 4942173"