u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞
細(xì)胞名稱:u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞(STR鑒定正確)
細(xì)胞別稱: U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG
細(xì)胞簡稱:u251細(xì)胞株
產(chǎn)品貨號:TCH-C366
規(guī)格: 1×10?cells/ T25 培養(yǎng)瓶
價格:1000元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來源:人
組織來源:神經(jīng)膠質(zhì)
特征:神經(jīng)膠質(zhì)細(xì)胞瘤
細(xì)胞形態(tài):成纖維細(xì)胞樣
生長特性:貼壁生長
培養(yǎng)體系:DMEM-H+10%FBS+1%P/S
u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞 配套培養(yǎng)基貨號: TCH-G366
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲存
質(zhì)量檢測:細(xì)菌、真菌、支原體檢測均為陰性
u251細(xì)胞株-人膠質(zhì)瘤細(xì)胞 分離于膠質(zhì)母細(xì)胞瘤組織。
參考文獻(xiàn):
"Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
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Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)
Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.
Origin of the U87MG glioma cell line: good news and bad news.
Sci. Transl. Med. 8:354re3-354re3(2016)
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)"
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