CHOK1細(xì)胞

1957年，PuckTT從成年中國(guó)倉(cāng)鼠卵巢的活檢組織建立了CHO細(xì)胞，CHOK1細(xì)胞是CHO的一個(gè)亞克隆。CHOK1細(xì)胞的生長(zhǎng)需要。 

細(xì)胞名稱：倉(cāng)鼠卵巢細(xì)胞

細(xì)胞簡(jiǎn)稱：CHO-K1

細(xì)胞別稱：CHO K1; CHOK1; CHO cell clone K1; GM15452

產(chǎn)品貨號(hào)：TCO-C005

規(guī)格： 1X10^6

種屬來(lái)源：倉(cāng)鼠

組織來(lái)源：卵巢

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：F12K+10%FBS+1%P/S

配套培養(yǎng)基貨號(hào)：TCO-G005

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

  CHOK1細(xì)胞為轉(zhuǎn)化細(xì)胞系，   在工業(yè)生產(chǎn)上應(yīng)用較多，  CHOK1細(xì)胞 染色體分布頻率是2n=22，系亞二倍體細(xì)胞。海星細(xì)胞庫(kù)保存CHO-K1細(xì)胞株，編號(hào)為TCO-C005，被廣泛地用于重組DNA蛋白的表達(dá)。

參考文獻(xiàn)：

Puck TT, et al. Genetics of somatic mammalian cells III. Long-term c*tion of euploid cells from human and animal subjects. J. Exp. Med. 108: 945-956, 1958. PubMed:

Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc. Natl. Acad. Sci. USA 53: 288-293, 1965. PubMed:

Kao FT, Puck TT. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60: 1275-1281, 1968. PubMed: 5244736

Kao FT, Puck TT. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: 513-524, 1967. PubMed: 6068403

Kao FT, et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: 312-314, 1969. PubMed: 5813351

Kao F, et al. Genetics of somatic mammalian cells. X. Complementation analysis of glycine-requiring mutants. Proc. Natl. Acad. Sci. USA 64: 1284-1291, 1969. PubMed: 5271752

Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365