EMT-6細(xì)胞-小鼠乳腺癌細(xì)胞

細(xì)胞名稱：EMT-6細(xì)胞-小鼠乳腺癌細(xì)胞  

細(xì)胞別稱： EMT-6; EMT6細(xì)胞,Experimental Mammary Tumour-6

貨號：TCM-C728

規(guī)格： 1X10^6/ t25細(xì)胞培養(yǎng)瓶

價格：目錄價1500元 (干冰運(yùn)輸需另加200元干冰費(fèi))

細(xì)胞簡稱：emt6細(xì)胞

產(chǎn)品貨號：TCM-C728

種屬來源：小鼠

組織來源：乳腺

特征：乳腺癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長特性：貼壁生長

培養(yǎng)體系：Waymouth's MB 752/1+15%FBS+1%Glutamax+1%P/S

EMT-6細(xì)胞-小鼠乳腺癌細(xì)胞 配套培養(yǎng)基貨號：TCM-G728

傳代比例：1:4-1:8，每2-3天換液一次

傳代周期：24-48 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

  EMT-6是在BALB/cCRGL小鼠的增生性乳腺泡結(jié)節(jié)植入后發(fā)生的可移植小鼠乳腺癌中建立的。所得到的腫瘤系(命名為KHJJ)在BALB/cKa小鼠中繁殖，動物傳代25次后適應(yīng)組織培養(yǎng)，命名為EMT。EMT6是1971年在斯坦福大學(xué)分離的EMT克隆分離株。

"Rockwell SC, et al. Characteristics of a serially transplanted mouse mammary tumor and its tissue-culture-adapted derivative. J. Natl. Cancer Inst. 49: 735-749, 1972. PubMed: 4647494

Palom Y, et al. Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells. Chem. Res. Toxicol. 13: 479-488, 2000. PubMed:

Collingridge DR, Rockwell S. Pentoxifylline improves the oxygenation and radiation response of BA1112 rat rhabdomyosarcomas and EMT6 mouse mammary carcinomas. Int. J. Cancer 90: 256-264, 2000. PubMed:

Rockwell S, Kelley M. RSR13, a synthetic allosteric modifier of hemoglobin, as an adjunct to radiotherapy: preliminary studies with EMT6 cells and tumors and normal tissues in mice. Radiat. Oncol. Investig. 6: 199-208, 1998. PubMed: 9822166

Rockwell S. In vivo-in vitro tumor systems: new models for studying the response of tumors to therapy. Lab. Anim. Sci. 27: 831-851, 1977. PubMed: 338981

Rockwell STumor-Cell SurvivalIn: Rockwell STumor models in cancer researchTotowa, New JerseyHumana Press Inc.617-631.

EMT6 was established from a transplantable murine mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule. The resulting tumor line (named KHJJ) was propagated in BALB/cKa mice and adapted to tissue culture after the 25th animal passage, and the cell line was named EMT. EMT6 is a clonal isolate of EMT isolated in 1971 at Stanford University. The EMT6 cell line can be grown either in animals as a tumor or in tissue culture. Cells derived from tumors have a reported in vitro plating efficiency of 30%. Cell grown in tissue culture reportedly have a plating efficiency of 70%.