詳細(xì)介紹

概述
在實(shí)際的蛋白分離、分析工作中，廣大科研工作者經(jīng)常會(huì)遇到某些疏水性蛋白及聚合物改性蛋白在常規(guī)SEC柱上出峰狀態(tài)與理論不符的情況。針對(duì)此種情況，賽分科技在SRT系列色譜柱的基礎(chǔ)上，專門推出針對(duì)該類樣品的Sepax SRT-C SEC系列色譜柱。該鍵合固定相采用的表面修飾技術(shù)，通過(guò)在高純度的硅膠基質(zhì)上鍵合均勻的納米厚度中性親水涂層而制備得到。由于采用可控的化學(xué)修飾技術(shù)，確保了填料批間有著可靠的重現(xiàn)性。SEC填料采用化學(xué)鍵合技術(shù)，表面親水涂層覆蓋*，因此不僅具有優(yōu)異的穩(wěn)定性，而且對(duì)蛋白等生物樣品的非特異性吸附作用也非常小。同時(shí)，大的孔體積保證了高的載樣量及優(yōu)異的分辨率。

技術(shù)參數(shù)：

固定相	SRT-C SEC-150	SRT-C SEC-300	SRT-C SEC- 500
材料	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica	Neutral, hydrophilic film bonded silica
顆粒大小	5 mm	5 mm	5 mm
孔徑 (Å)	~ 150	~ 300	~ 500
蛋白質(zhì)分子量范圍	500 - 150,000	5,000 – 1,250,000	15,000 – 5,000,000
水溶性聚合物分子量范圍	500-25,000	1,000-100,000	25,000-500,000
pH 穩(wěn)定性	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)	2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
反壓(psi for a 7.8x300 mm)	~ 700	~ 700	~ 700
壓力 (psi)	~ 4,500	~ 3,500	~ 3,000
鹽濃度范圍	20 mM - 2.0 M	20 mM - 2.0 M	20 mM - 2.0 M
使用溫度 (^oC)	~ 80	~ 80	~ 80
流動(dòng)相兼容性	Aqueous and organic	Aqueous and organic	Aqueous and organic

訂購(gòu)信息

Sepax SRT-C SEC-150

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號(hào)
SRT-C SEC-150	5um	150 Å	21.5x600mm	235150-21560
SRT-C SEC-150	5um	150 Å	21.5x300mm	235150-21530
SRT-C SEC-150	5um	150 Å	7.5x600mm	235150-7560
SRT-C SEC-150	5um	150 Å	7.8x300mm	235150-7830
SRT-C SEC-150	5um	150 Å	7.8x150mm	235150-7815
SRT-C SEC-150	5um	150 Å	7.8x50 (Guard)	235150-7805
SRT-C SEC-150	5um	150 Å	4.6x300mm	235150-4630
SRT-C SEC-150	5um	150 Å	4.6x150mm	235150-4615
SRT-C SEC-150	5um	150 Å	4.6x50 (Guard)	235150-4605

Sepax SRT-C SEC-300

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號(hào)
SRT-C SEC-300	5um	300 Å	21.5x600mm	235300-21560
SRT-C SEC-300	5um	300 Å	21.5x300mm	235300-21530
SRT-C SEC-300	5um	300 Å	7.5x600mm	235300-7560
SRT-C SEC-300	5um	300 Å	7.8x300mm	235300-7830
SRT-C SEC-300	5um	300 Å	7.8x150mm	235300-7815
SRT-C SEC-300	5um	300 Å	7.8x50 (Guard)	235300-7805
SRT-C SEC-300	5um	300 Å	4.6x300mm	235300-4630
SRT-C SEC-300	5um	300 Å	4.6x150mm	235300-4615
SRT-C SEC-300	5um	300 Å	4.6x50 (Guard)	235300-4605

Sepax SRT-C SEC-500

產(chǎn)品名稱	粒度	孔徑	規(guī)格	訂貨號(hào)
SRT-C SEC-500	5um	500 Å	21.5x600mm	235500-21560
SRT-C SEC-500	5um	500 Å	21.5x300mm	235500-21530
SRT-C SEC-500	5um	500 Å	7.5x600mm	235500-7560
SRT-C SEC-500	5um	500 Å	7.8x300mm	235500-7830
SRT-C SEC-500	5um	500 Å	7.8x150mm	235500-7815
SRT-C SEC-500	5um	500 Å	7.8x50 (Guard)	235500-7805
SRT-C SEC-500	5um	500 Å	4.6x300mm	235500-4630
SRT-C SEC-500	5um	500 Å	4.6x150mm	235500-4615
SRT-C SEC-500	5um	500 Å	4.6x50 (Guard)	235500-4605