詳細(xì)介紹
概述
Carbomix系列色譜柱專為高效分離制備水溶性或部分水溶性的有機(jī)化合物而設(shè)計(jì),可用于分離糖類、有機(jī)酸、多肽和細(xì)胞代謝物中的生物有機(jī)小分子。Carbomix系列固定相是在低交聯(lián)度(5%、8%和10%)的聚苯乙烯/二乙烯基苯(PS/DVB)顆粒(5、10 μm)表面鍵合磺酸基團(tuán)(-SO3H),即為Carbomix H-NP填料;若在此填料上繼續(xù)絡(luò)合不同的離子則形成不同類型的填料。如絡(luò)合離子為鈣離子(Ca2+),則為Carbomix Ca-NP填料;如絡(luò)合離子為鉛離子(Pb2+),則為Carbomix Pb-NP填料;如絡(luò)合離子為鉀離子(K+),則為Carbomix K-NP填料;如絡(luò)合離子為鈉離子(Na+),則為Carbomix Na-NP填料。
技術(shù)參數(shù)
Phase | Carbomix H-NP | Carbomix Ca-NP | Carbomix Pb-NP | Carbomix K-NP | Carbomix Na-NP | |||
Support | Non-porous PS/DVB | Non-porous PS/DVB | Non-porous PS/DVB | Non-porous PS/DVB | Non-porous PS/DVB | |||
Particle size (mm) | 5, 10 | 5, 10 | 5, 10 | 5, 10 | 5, 10 | |||
Crosslinkage | 5%, 8% and 10% | 5%, 8% and 10% | 5%, 8% and 10% | 5%, 8% and 10% | 5%, 8% and 10% | |||
Stationary phase | -SO3H | -(SO3)2Ca | -(SO3)2Pb | -SO3K | -SO3Na | |||
pH stability | 1 – 3 | 5 – 9 | 5 – 9 | 5 – 9 | 5 – 9 | |||
Maximum backpressure (psi) (1) | 10% crosslinking | 10 mm | 1,500 | 1,500 | 1,500 | 1,500 | 1,500 | |
5 mm | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | |||
8% crosslinking | 10 mm | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | ||
5 mm | 800 | 800 | 800 | 800 | 800 | |||
5% crosslinking | 10 mm | 600 | 600 | 600 | 600 | 600 | ||
5 mm | 400 | 400 | 400 | 400 | 400 | |||
Typical phase | 2.5 mM H2SO4 or 0.1% H3PO4 | H2O | H2O | H2O | H2O | |||
Typical flow rate (mL/min)(2) | A 7.8x300mm column | 0.4-0.8 | 0.4-0.8 | 0.4-0.8 | 0.4-0.8 | 0.4-0.8 | ||
A 4.6x300mm column | 0.1-0.30 | 0.1-0.30 | 0.1-0.30 | 0.1-0.30 | 0.1-0.30 | |||
Maximum temperature (oC) | 85 | 85 | 85 | 85 | 85 | |||
(1) For a 7.8 ′ 300 mm column at maximum temperature, and (2) the back pressure from the instrument being subtracted.
(2) For 5mm, 5% crosslinking resin, the flow rate for a 7.8x300mm column is 0.1 to 0.15 mL/min.
典型應(yīng)用
Carbomix樹脂和色譜柱為食品和飲料中糖類、醇類、有機(jī)酸的分離,以及生物化學(xué)、生物醫(yī)藥、生物技術(shù)等領(lǐng)域的應(yīng)用提供了許多便利。
有機(jī)酸和醇類分析,包括糖類與有機(jī)酸、乙醇、乙二醇和發(fā)酵產(chǎn)品等。
糖類分析,包括甜菜糖、糖蜜、玉米糖漿、戊糖、纖維水解物、寡糖、葡萄糖、半乳糖、蔗糖、果糖等。
典型的工業(yè)應(yīng)用領(lǐng)域包括:
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訂購(gòu)信息
Sepax Carbomix H-NP
產(chǎn)品名稱 | 粒度 | 交聯(lián)度 | 規(guī)格 | 訂貨號(hào) |
Carbomix H-NP | 10 μm | 5% | 4.6 ′ 50 mm | 261005-4605 |
Carbomix H-NP | 10 μm | 5% | 4.6 ′ 250 mm | 261005-4625 |
Carbomix H-NP | 10 μm | 5% | 4.6 ′ 300 mm | 261005-4630 |
Carbomix H-NP | 10 μm | 5% | 7.8 ′ 50 mm | 261005-7805 |
Carbomix H-NP | 10 μm | 5% | 7.8 ′ 100 mm | 261005-7810 |
Carbomix H-NP | 10 μm | 5% | 7.8 ′ 300 mm | 261005-7830 |
Carbomix H-NP | 10 μm | 5% | 10 ′ 300 mm | 261005-10030 |
Carbomix H-NP | 10 μm | 8% | 4.6 ′ 50 mm | 261008-4605 |
Carbomix H-NP | 10 μm | 8% | 4.6 ′ 250 mm | 261008-4625 |
Carbomix H-NP | 10 μm | 8% | 4.6 ′ 300 mm | 261008-4630 |
Carbomix H-NP | 10 μm | 8% | 7.8 ′ 50 mm | 261008-7805 |
Carbomix H-NP | 10 μm | 8% | 7.8 ′ 100 mm | 261008-7810 |
Carbomix H-NP | 10 μm | 8% | 7.8 ′ 300 mm | 261008-7830 |
Carbomix H-NP | 10 μm | 8% | 10 ′ 300 mm | 261008-10030 |
Carbomix H-NP | 10 μm | 10% | 4.6 ′ 50 mm | 261010-4605 |
Carbomix H-NP | 10 μm | 10% | 4.6 ′ 250 mm | 261010-4625 |
Carbomix H-NP | 10 μm | 10% | 4.6 ′ 300 mm | 261010-4630 |
Carbomix H-NP | 10 μm | 10% | 7.8 ′ 50 mm | 261010-7805 |
Carbomix H-NP | 10 μm | 10% | 7.8 ′ 100 mm | 261010-7810 |
Carbomix H-NP | 10 μm | 10% | 7.8 ′ 300 mm | 261010-7830 |
Carbomix H-NP | 10 μm | 10% | 10 ′ 300 mm | 261010-10030 |
Sepax Carbomix Ca –NP
產(chǎn)品名稱 | 粒度 | 交聯(lián)度 | 規(guī)格 | 訂貨號(hào) |
Carbomix Ca-NP | 10 μm | 5% | 4.6 ′ 50 mm | 251005-4605 |
Carbomix Ca-NP | 10 μm | 5% | 4.6 ′ 250 mm | 251005-4625 |
Carbomix Ca-NP | 10 μm | 5% | 4.6 ′ 300 mm | 251005-4630 |
Carbomix Ca-NP | 10 μm | 5% | 7.8 ′ 50 mm | 251005-7805 |
Carbomix Ca-NP | 10 μm | 5% | 7.8 ′ 100 mm | 251005-7810 |
Carbomix Ca-NP | 10 μm | 5% | 7.8 ′ 300 mm | 251005-7830 |
Carbomix Ca-NP | 10 μm | 5% | 10 ′ 300 mm | 251005-10030 |
Carbomix Ca-NP | 10 μm | 8% | 4.6 ′ 50 mm | 251008-4605 |
Carbomix Ca-NP | 10 μm | 8% | 4.6 ′ 250 mm | 251008-4625 |
Carbomix Ca-NP | 10 μm | 8% | 4.6 ′ 300 mm | 251008-4630 |
Carbomix Ca-NP | 10 μm | 8% | 7.8 ′ 50 mm | 251008-7805 |
Carbomix Ca-NP | 10 μm | 8% | 7.8 ′ 100 mm | 251008-7810 |
Carbomix Ca-NP | 10 μm | 8% | 7.8 ′ 300 mm | 251008-7830 |
Carbomix Ca-NP | 10 μm | 8% | 10 ′ 300 mm | 251008-10030 |
Carbomix Ca-NP | 10 μm | 10% | 4.6 ′ 50 mm | 251010-4605 |
Carbomix Ca-NP | 10 μm | 10% | 4.6 ′ 250 mm | 251010-4625 |
Carbomix Ca-NP | 10 μm | 10% | 4.6 ′ 300 mm | 251010-4630 |
Carbomix Ca-NP | 10 μm | 10% | 7.8 ′ 50 mm | 251010-7805 |
Carbomix Ca-NP | 10 μm | 10% | 7.8 ′ 100 mm | 251010-7810 |
Carbomix Ca-NP | 10 μm | 10% | 7.8 ′ 300 mm | 251010-7830 |
Carbomix Ca-NP | 10 μm | 10% | 10 ′ 300 mm | 251010-10030 |
注:以上為常備規(guī)格訂貨信息,其它規(guī)格或特殊定制產(chǎn)品,電詢。