上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-MAP3K9抗體,我公司銷(xiāo)售國(guó)內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標(biāo)記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動(dòng)物血清等產(chǎn)品,歡迎廣大新老客戶(hù)前來(lái)咨詢(xún)訂購(gòu),訂購(gòu):或。
絲裂原活化蛋白激酶3K9抗體,Anti-MAP3K9抗體簡(jiǎn)單介紹:
中文名稱(chēng):絲裂原活化蛋白激酶3K9抗體
英文名稱(chēng):Anti-MAP3K9抗體
別 名:MLK1; M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; PRKE1.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
背景介紹:Mixed lineage kinases are a family of protein kinases sharing two leucine zipper-like motifs, which are known to mediate protein dimerization, and a kinase domain whose primary structure is similar to both the tyrosine-specific and the serine/threonine-specific kinase classes. Members of the mixed-lineage kinase (MLK) family include MLK1, MLK2, MLK3 and dual leucine zipper kinase, also designated DLK. MLKs are expressed in neuronal cells where they are likely to interact between Rac1/Cdc42, MKK4 and MKK7 in death signaling. The human MLK1 gene maps to chromosome 14q24.3-q31 and is expressed in epithelial tumor cell lines of the colon, breast, and esophagus. The human MLK2 gene maps to chromosome 19 q13.2. and encodes a predicted 954 amino acid, src homology 3 (SH3) domain-containing protein. The human MLK3 gene maps to chromosome 11q13.1-13.3 and encodes a 847 amino acid, SH3 domain- and proline rich region-containing protein. Apoptosis mechanisms rely on MLKs as an upstream intermediate of mitochondrial cytochrome c release and caspase activation.
規(guī)格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來(lái)源:本公司抗體有兔來(lái)源和鼠來(lái)源等
克隆類(lèi)型:兔來(lái)源為多抗,鼠來(lái)源為單抗
產(chǎn)品類(lèi)型:一抗
絲裂原活化蛋白激酶3K9抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融,保質(zhì)期一年。
絲裂原活化蛋白激酶3K9抗體,Anti-MAP3K9抗體免疫組化問(wèn)題解答
1、染色過(guò)強(qiáng)?
原 因:抗體濃度過(guò)高或孵育時(shí)間過(guò)長(zhǎng)
解決方法:降低抗體滴度、抗體孵育時(shí)間:室溫1小時(shí)或4度過(guò)夜
原 因:孵育溫度過(guò)高超過(guò)37度
解決方法:一般在室溫20-28度
原 因:DAB顯色時(shí)間過(guò)長(zhǎng)或濃度過(guò)高
解決方法:顯色時(shí)間不超過(guò)5-12分鐘,以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色?
原 因:操作過(guò)程中沖洗不充分
解決方法:每步?jīng)_洗3次每次5分鐘
原 因:組織中含過(guò)氧化物酶未阻斷
解決方法:可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因:組織中含內(nèi)原性*
解決方法:正常非免疫動(dòng)物血清再封閉
原 因:血清蛋白封閉不充分
解決方法:血清蛋白封閉不充分
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