上海研盟生物科技有限公司Anti-SerpinB2抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-SerpinB2 antibody
中文名稱:纖溶酶原激活物抑制因子2/血漿酶原激活酶抑制因子-2抗體
血漿酶原激活酶抑制因子-2抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
血漿酶原激活酶抑制因子-2抗體,Anti-SerpinB2抗體抗原的分類:
一. 免疫學分類:
(1)*抗原、半抗原;
(2)胸腺依賴抗原與非依賴抗原。
二. 臨床分類:
(1)外源性抗原:微生物、花粉等;
(2)內源性抗原:隱蔽的自身抗原、腫瘤相關抗原等;
(3)同種異型抗原:人類白細胞抗原、血型抗原;
(4)異嗜性抗原:人與其他動物、植物等之間存在的共同抗原。
★研盟生物★血漿酶原激活酶抑制因子-2抗體,Anti-SerpinB2抗體
免疫印跡試驗常見的問題及原因:1、沒有條帶
可能存在的原因 | 解決方法 |
抗體不足 | 抗體與蛋白的親和不高,加大抗體的濃度;抗體活性降低,斑點實驗。 |
蛋白量不足 | 增加蛋白的上樣量;其他途徑測定蛋白含量;添加一個陽性對照;斑點實驗 |
蛋白轉移失敗 | PVDF:甲醇激活,NC和PVDF在轉膜液中浸泡;保持膜與膠之間的無氣泡接觸。 |
蛋白轉移率低 | 優(yōu)化轉膜時間,高分子量蛋白可能需要更長的時間,轉膜之后可以用麗春紅染色,并用預染的maker作為指示。 |
蛋白轉移透膜 | 降低電壓或者時間當分子量小于10kd時 |
等電點大于9 | 使用更高ph值的溶液體系(pH10.5) |
二抗使用錯誤 | 二抗的種屬錯誤 |
過期的抗體 | 購買新抗體 |
抗體的不正確儲存 | 依據(jù)生產(chǎn)商手冊,避免反復凍融 |
抗體的不正確儲存 | 避免二抗中含有疊氮鈉,它能催眠HRP信號 |
一抗結合時間不足 | 4度過夜孵育 |
2、微弱的條帶(weak single)
可能存在的原因 | 解決辦法 |
蛋白與抗體結合率低 | 較少洗膜次數(shù)或者縮短洗膜時間; 降低洗膜液中NaCl的濃度(0.15M-0.5M); 降低抗體稀釋液中NaCl的濃度(0.15M-0.5M) |
抗體量不足 | 抗體和蛋白的結合較低,可將抗體濃度調整至初始濃度的2-4倍 |
蛋白量不足 | 增加蛋白上樣量 |
酶和底物時效 | 將酶和底物進行反應,觀察現(xiàn)象,出現(xiàn)異常,則需要重新標記或者購買 |
過期或者陳舊的ECL發(fā)光液 | 更換的新的ECL發(fā)光液 |
脫脂奶粉可能會掩蓋一些抗原 | 降低牛奶的百分比或者用BSA代替 |
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相關標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
簡單介紹:
產(chǎn)品別名:Serpin B2; Serpinb2; SerpinB2; plasminogen activator inhibitor 2; HsT1201; Monocyte Arg serpin; Monocyte Arg-serpin; PAI2A; PAI; PAI-2; PAI 2; PAI2; Placental plasminogen activator inhibitor; PAI2_RAT; PLANH2; Plasminogen activator inhibitor 2; Plasminogen activator inhibitor 2 type A; Serpin peptidase inhibitor clade B (ovalbumin) member 2; Urokinase inhibitor.
抗體來源:Rabbit or Mouse
保質期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:SerpinB2 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in the placenta, and given the name PAI-2 because of the ability to inhibit urokinase plasminogen activator (u-PA) at low micromolar efficiency. SerpinB2 also inhibits tissue plasminogen activator (t-PA), but with micromolar efficiency, and PAI-1 is much more efficient that SerpinB2 on both forms of plasminogen activator. The structure of PAI-2 is not terribly similar to PAI-1, however, which is an E clade serpin.
SerpinB2 is made by many cell types, and is found intracellularly as an unglycosylated kDa protein, and secreted as a 60 kDa protein. SerpinB2 is found in saliva, secreted by gingival fibroblasts, and in the skin. SerpinB2 levels are elevated in serum during pregnancy, and in leukemia, breast cancer and ovarian cancer, although it was lowered in some cancers.A shorter SerpinB2 isoform of 382 amino acids, has been reported, with a predicted mass of 43.1 kDa and a pI of 5.69. The shorter form has a deletion just after the start of the mature protein, but it is unclear what the relative production and distribution is for the shorter sequence.
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