上海研盟生物有限公司*品質(zhì)供應Anti-LAMR1(5D4)抗體，我公司銷售國內(nèi)外科研產(chǎn)品，如生物試劑、生化試劑，一抗二抗，各種標記抗體，Elisa酶聯(lián)免疫試劑盒，放免試劑盒，培養(yǎng)基，各種動物血清等產(chǎn)品，歡迎廣大新老客戶前來咨詢訂購，訂購：或。

層粘連蛋白受體1單克隆抗體,Anti-LAMR1(5D4)抗體簡單介紹：

【中文名稱】：層粘連蛋白受體1單克隆抗體

【英文名稱】：Anti-LAMR1(5D4)抗體

【英文別名】：37LRP; P37-kDa laminin receptor precursor; MGr1-Ag; laminin receptor 1; 67kD, ribosomal protein SA; 34/67 kDa laminin receptor; 40S ribosomal protein SA; 67LR; Colon carcinoma laminin-binding protein; LAMBR; Laminin Receptor 1; LAMR 1; LAMR1; LRP; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; RPSA; 67kD laminin receptor.  

【規(guī)  格】：0.1ml/0.2ml

【相關標記】：Alexa Fluor 350、Alexa Fluor 488、Alexa Fluor 555、Alexa Fluor 647、AP、APC、Biotin、Cy3、Cy5、Cy5.5、Cy7、FITC、Gold、HRP、PE、PE-Cy3、PE-CY5、PE-CY5.5、PE-CY7、RBITC.【濃    度】：1mg/1ml

【抗體來源】：Rabbit or Mouse or Goat 

【克隆類型】：polyclonal or monoclonal

【產(chǎn)品類型】：一抗

【產(chǎn)品應用】：可以用于做石蠟切片免疫組化，冰凍切片免疫組化，Elisa，WB，免疫熒光、流式細胞術等實驗。

層粘連蛋白受體1單克隆抗體【背景介紹】：Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

層粘連蛋白受體1單克隆抗體,Anti-LAMR1(5D4)抗體免疫組化操作流程：

（一） 儀器設備
1. 18cm不銹鋼高壓鍋或電爐或用微波爐.
2. 水浴鍋
（二） 試 劑
1. PBS緩沖液（ph7.2―7.4）：NaC137mmol/L，KCl 2.7mmol/L ,Na2HPO4 4.3mmol/L,
KH2PO4 1.4mmol/L.
2．0.01mol/L檸檬酸鈉緩沖液（CB,ph6.0,1000ml）：檸檬酸三鈉3g,檸檬酸0.4g。
3．0.5mol/L EDTA緩沖液（ph8.0）：700ml水中溶解186.1g EDTA&
8226;2H2O,用10mmol/L
NaOH調(diào)至ph8.0,加水至1000ml.
4. 1mol/L的TBS緩沖液（ph8.0）：在800ml水中溶解121gTris堿，用1N的HCl調(diào)至ph8.0,加水1000ml。
5. 酶消化液：a. 0.1%*：用0.1%CaCl 12（ph7.8）配制。b.0.4%*液：用0.1N的HCl配制。
6. 3%甲醇―H2O2溶液：用30%H2O2和80%甲醇溶液配制
7. 風裱劑：a. 甘油和0.5mmol/L碳酸鹽緩沖液（ph9.0–9.5）等量混合
b 油和TBS（PBS）配制
8．TBS/PBS PH9.0–9.5,適用于熒光纖維鏡標本;ph7.0-7.4適合光學纖維標本。
（三） 操作流程
1 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片

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相關標記抗體：HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體

層粘連蛋白受體1單克隆抗體,Anti-LAMR1(5D4)抗體相關優(yōu)質(zhì)產(chǎn)品：

Cyclin A  周期素A抗體
Cyclin B1  周期素B1抗體
Cyclin C  周期素C抗體
Cyclin D1  周期素D1抗體
Cyclin D2  周期素D2抗體
Cyclin D3(human, mo, rat, pig, cattle, dog, Rb)  周期素D3抗體
Cyclin E  周期素E/原癌基因抗體
Cyclin F  周期素F抗體
Cyclin G  周期素G抗體
Cyclin H  周期素H抗體
Cygb (cytoglobin)  細胞球蛋白抗體
CYP1A1(Cytochrome p450 1A1)  細胞色素P450 1A1抗體
P450 1A2(Cytochrome P450 1A2)  細胞色素P450 1A2抗體
CYP3A4(Cytochrome p450 3A4)  抗細胞色素P450 3A4抗體
CYP19(Cytochrome p19)  細胞色素P450 19抗體
CYP21(Cytochrome P450 21; cytochrome P450, family 21, subfamily A, polypeptide 2)  21-羥化酶抗體
CYP450(Cytochrome P450 monooxygenase)  細胞色素P450單氧化酶抗體
Cyr61/IGFBP10/CCN1(cysteine rich 61)  富半*肝素結(jié)合蛋白61抗體
CYP2E1(cytochrome P450 2E1)  抗細胞色素P450ⅡE1抗體
Cytochrome C  細胞色素 C抗體
DRD1 receptor (dopamine D1 receptor)  多巴胺受體-D1抗體
DRD2 receptor(dopamine D2 receptor)  多巴胺受體-D2抗體
DRD3 receptor(dopamine D3 receptor)  多巴胺受體-D3抗體