產(chǎn)品介紹 | |||
品名 | Rabbit anti-calretinin | ||
貨號 | 7697 | ||
規(guī)格 | 200 µl、500 µl、1mL | ||
克隆性 | 多克隆 | ||
共軛 | 非共軛 | ||
反應性 | 人、鼠、leopard danio | ||
應用 | 免疫組織化學、免疫組織化學-石蠟切片、免疫組織化-冷凍切片 | ||
宿主動物 | 兔 | ||
抗體形式 | 血清凍干 | ||
產(chǎn)品形式 | 凍干全血清(不含防腐劑) | ||
復溶 | 用200µl雙蒸餾水 | ||
產(chǎn)品描述 | Rabbit anti-calretinin通過用在N-末端含有6-his標簽的重組人鈣視網(wǎng)膜蛋白免疫,在兔中產(chǎn)生針對鈣視網(wǎng)膜蛋白的抗血清。該抗體的特異性和效力進行了評估:a)通過生Wu素-阿維丁標記4%多聚甲醛固定腦的冷凍態(tài)、振動體和鏈烷烴切片,以及b)通過免疫印跡的免疫酶標記 | ||
稀釋 | 疫組織化學:在多聚甲醛(4%)或福爾馬林固定的組織、冷凍狀態(tài)或石蠟切片上進行1:2’000-1:5’000。 免疫印跡:1:1’000-1:2’000。 對于免疫組織化學和免疫印跡,使用親和素-生Wu素法測定滴度。我們建議通過滴定實驗來確定稀釋度。 | ||
儲存 | 重構(gòu)后,冷凍成小份(如1µl),并保持在-80°C(或至少-20°C)。使用時,保持在4°C(含0.01%疊Dan化鈉)。避免反復冷凍和解凍。 |
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