HE037
豚鼠神經(jīng)肽B（NKB）ELISA試劑盒
Guinea pig Neurokinins B,NKB ELISA Kit
樣本處理方法：
血清樣本：室溫血液自然凝固10-20分鐘，離心 20 分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集
上清，保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。
血漿樣本：應(yīng)根據(jù)標(biāo)本的要求選擇EDTA或檸檬酸鈉作為抗凝劑，混合10-20分鐘后，離心
20 分鐘左右（2000-3000 轉(zhuǎn)/分）。仔細(xì)收集上清，保存過程中如有沉淀形成，應(yīng)該再次
離心。
尿液樣本：用無菌管收集，離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清，保存過程
中如有沉淀形成，應(yīng)再次離心。胸腹水、腦脊液參照實(shí)行。
豚鼠神經(jīng)肽B（NKB）ELISA試劑盒細(xì)胞培養(yǎng)上清樣本：檢測分泌性的成份時(shí)，用無菌管收集。離心20分鐘左右（2000-3000 轉(zhuǎn)/
分）。仔細(xì)收集上清。檢測細(xì)胞內(nèi)的成份時(shí)，用PBS（PH7.2-7.4）稀釋細(xì)胞懸液，細(xì)胞
濃度達(dá)到100萬/ml 左右。通過反復(fù)凍融，以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心20分
鐘左右（2000-3000 轉(zhuǎn)/分）。仔細(xì)收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。
組織標(biāo)本：切割標(biāo)本后，稱取重量。加入一定量的 PBS，PH7.4。用液氮迅速冷凍保存?zhèn)?br />用。標(biāo)本融化后仍然保持2-8℃的溫度。加入一定量的 PBS（PH7.4），用手工或勻漿器
將標(biāo)本勻漿充分。離心20分鐘左右（2000-3000轉(zhuǎn)/分）。仔細(xì)收集上清。分裝后一份待
檢測，其余冷凍備用。
標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上
進(jìn)行試驗(yàn)，可將標(biāo)本放于-20℃保存，但應(yīng)避免反復(fù)凍融，不能檢測含 NaN3 的樣品，因
NaN3抑制辣根過氧化物酶的（HRP）活性。
Specimen requirements
1. serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at
the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared,
Centrifugal again.
2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20
mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,
If precipitation appeared, Centrifugal again.
3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of
2000-3000 r.p.m.remove supernatant, If precipitation appeared, Centrifugal again.
The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4. cell culture supernatant-detect secretory components, collect sue a sterile
container,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove
supernatant,detect the composition of cells, Dilut cell suspension with PBS
（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw
cycles, damage cells and release of intracellular components, centrifugation 20-
min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation
appeared, Centrifugal again.
5. Tissue samples-After cutting samples, check the weight,add PBS（PH7.2-7.4）,
Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add
PBS（PH7.4）,Homogenized by hand or Grinders, centrifugation 20-min at the speed
of 2000-3000 r.p.m.remove supernatant.
6. extract as soon as possible after Specimen collection,and according to the
relevant literature, and should be experiment as soon as possible after the
extraction. If it can’t,specimen can be kept in -20 ℃ to preserve, Avoid
repeated freeze-thaw cycles.
7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
供應(yīng)商：上海華壹生物科技有限公司
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