牛*(Amylase)ELISA試劑盒實(shí)驗(yàn)原理：本試劑盒用于測(cè)定牛血清，血漿及相關(guān)液體樣本中*(Amylase)的活性。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中牛*(Amylase)水平。用純化的牛*(Amylase)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入*(Amylase)，再與HRP標(biāo)記的*(Amylase)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的*(Amylase)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中牛*(Amylase)濃度。
Bovine Amylase ELISA Kit
Principle of the assay:
This kit allows for the determination of Amylase in Bovine serum, blood plasma, and other biological fluids.Assay Bovine Amylase level in the sample，use Purified Bovine Amylase to coat microtiter plate wells, make solid-phase antibody, then add Amylase to wells, Combined Amylase antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Amylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
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