豬胰島素(INS)ELISA試劑盒
Porcine Insulin(INS)ELISA Kit
實(shí)驗(yàn)原理：
本試劑盒僅供研究使用，用于測定豬血清，血漿及相關(guān)液體樣本中胰島素(INS)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中豬胰島素(INS)水平。用純化的豬胰島素(INS)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入胰島素(INS)，再與HRP標(biāo)記的胰島素(INS)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的胰島素(INS)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中豬胰島素(INS)濃度。
Principle of the assay
The kit assay Porcine INS level in the sample，use Purified Porcine INS to coat microtiter plate wells, make solid-phase antibody, then add INS to wells, Combined INS which With HRP labeled ， become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應(yīng)商：上海華壹生物科技有限公司
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