小鼠促卵泡激素（FSH）酶聯(lián)免疫分析（ELISA）試劑盒
Mouse Follicle-stimulating hormone (FSH) ELISA Kit
實(shí)驗(yàn)原理：本試劑盒僅供研究使用，用于測(cè)定小鼠血清，血漿及相關(guān)液體樣本中促卵泡激素（FSH）的含量。應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中小鼠促卵泡激素（FSH）水平。用純化的小鼠促卵泡激素（FSH）抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入促卵泡激素（FSH），再與HRP標(biāo)記的FSH抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的小鼠促卵泡激素（FSH）呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計(jì)算樣品中小鼠促卵泡激素（FSH）濃度。
Principle of the assay
The kit assay Mouse FSH level in the sample，use Purified Mouse FSH antibody to coat microtiter plate wells, make solid-phase antibody, then add FSH to wells, Combined FSH which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA試劑盒供應(yīng)商：上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
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