人脂聯(lián)素酶聯(lián)免疫分析ELISA試劑盒實驗原理本試劑盒用于測定人血清，血漿及相關(guān)液體樣本中脂聯(lián)素（ADP)的含量。應(yīng)用雙抗體夾心法測定標(biāo)本中人脂聯(lián)素（ADP)水平。用純化的抗-脂聯(lián)素（ADP)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入脂聯(lián)素（ADP)，再與HRP標(biāo)記的脂聯(lián)素（ADP)抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的脂聯(lián)素（ADP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中人脂聯(lián)素（ADP)濃度。
Human Adiponectin (ADP) ELISA Kit
Principle of the assay
The kit assay Human ADP level in the sample，use Purified Human ADP antibody to coat microtiter plate wells, make solid-phase antibody, then add ADP to wells, Combined ADP which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
