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上海華壹生物科技有限公司
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閱讀:425發(fā)布時間:2016-4-13
兔α干擾素(IFN-α)酶聯(lián)免疫分析(ELISA)試劑盒
Rabbit Interferon α(IFN-α) ELISA Kit
實驗原理:本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中兔α干擾素(IFN-α)水平。用純化的兔α干擾素(IFN-α)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入α干擾素(IFN-α),再與HRP標(biāo)記的IFN-α抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的α干擾素(IFN-α)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計算樣品中兔α干擾素(IFN-α)濃度。
試劑盒組成:
試劑盒組成 | 48孔配置 | 96孔配置 | 保存 |
說明書 | 1份 | 1份 |
|
封板膜 | 2片(48) | 2片(96) |
|
密封袋 | 1個 | 1個 |
|
酶標(biāo)包被板 | 1×48 | 1×96 | 2-8℃保存 |
標(biāo)準(zhǔn)品:45ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃保存 |
標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃保存 |
酶標(biāo)試劑 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
樣品稀釋液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑A液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑B液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
終止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃保存 |
濃縮洗滌液 | (20ml×20倍)×1瓶 | (20ml×30倍)×1瓶 | 2-8℃保存 |
Principle of the assay
The kit assay Rabbit IFN-α level in the sample,use Purified Rabbit IFN-α antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-α to wells, Combined IFN-α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit | 48determinations | 96 determinations | Storage |
User manual | 1 | 1 |
|
Closure plate membrane | 2 | 2 |
|
Sealed bags | 1 | 1 |
|
Microelisa stripplate | 1 | 1 | 2-8℃ |
Standard:45ng/L | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
wash solution | (20ml×20 fold) ×1bottle | (20ml×30 fold) ×1bottle | 2-8℃ |
注:本產(chǎn)品僅供科研實驗使用,信息供參考,具體產(chǎn)品信息請來電或在線方式咨詢。
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
商鋪:http://www.hg1112.cn/st39446/
主營產(chǎn)品:生物試劑、ELISA試劑盒、對照品標(biāo)準(zhǔn)品、抗體抗原、動物血清、實驗室耗材、培養(yǎng)基、染色液等產(chǎn)品。
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