兔α干擾素(IFN-α)酶聯(lián)免疫分析（ELISA）試劑盒
Rabbit Interferon α(IFN-α) ELISA Kit
實驗原理：本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中兔α干擾素(IFN-α)水平。用純化的兔α干擾素(IFN-α)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入α干擾素(IFN-α)，再與HRP標(biāo)記的IFN-α抗體結(jié)合，形成抗體-抗原-酶標(biāo)抗體復(fù)合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的α干擾素(IFN-α)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（OD值），通過標(biāo)準(zhǔn)曲線計算樣品中兔α干擾素(IFN-α)濃度。
試劑盒組成：
試劑盒組成 | 48孔配置 | 96孔配置 | 保存 |
說明書 | 1份 | 1份 | |
封板膜 | 2片（48） | 2片（96） | |
密封袋 | 1個 | 1個 | |
酶標(biāo)包被板 | 1×48 | 1×96 | 2-8℃保存 |
標(biāo)準(zhǔn)品：45ng/L | 0.5ml×1瓶 | 0.5ml×1瓶 | 2-8℃保存 |
標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 | 1.5ml×1瓶 | 2-8℃保存 |
酶標(biāo)試劑 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
樣品稀釋液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑A液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
顯色劑B液 | 3 ml×1瓶 | 6 ml×1瓶 | 2-8℃保存 |
終止液 | 3ml×1瓶 | 6ml×1瓶 | 2-8℃保存 |
濃縮洗滌液 | （20ml×20倍）×1瓶 | （20ml×30倍）×1瓶 | 2-8℃保存 |
Principle of the assay
The kit assay Rabbit IFN-α level in the sample，use Purified Rabbit IFN-α antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-α to wells, Combined IFN-α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit | 48determinations | 96 determinations | Storage |
User manual | 1 | 1 | |
Closure plate membrane | 2 | 2 | |
Sealed bags | 1 | 1 | |
Microelisa stripplate | 1 | 1 | 2-8℃ |
Standard：45ng/L | 0.5ml×1 bottle | 0.5ml×1 bottle | 2-8℃ |
Standard diluent | 1.5ml×1 bottle | 1.5ml×1 bottle | 2-8℃ |
HRP-Conjugate reagent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Sample diluent | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution A | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Chromogen Solution B | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
Stop Solution | 3ml×1 bottle | 6ml×1 bottle | 2-8℃ |
wash solution | （20ml×20 fold） ×1bottle | （20ml×30 fold） ×1bottle | 2-8℃ |
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上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
