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上海華壹生物科技有限公司


當(dāng)前位置:上海華壹生物科技有限公司>技術(shù)文章>兔α干擾素(IFN-α)酶聯(lián)免疫(ELISA)試劑盒組成

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商鋪產(chǎn)品:9954條

所在地區(qū):上海上海市

聯(lián)系人:銷售部

技術(shù)文章

兔α干擾素(IFN-α)酶聯(lián)免疫(ELISA)試劑盒組成

閱讀:425發(fā)布時間:2016-4-13

α干擾素(IFN-α)酶聯(lián)免疫分析(ELISA試劑盒

Rabbit Interferon α(IFN-α) ELISA Kit

實驗原理:本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中α干擾素(IFN-α)水平。用純化的α干擾素(IFN-α)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入α干擾素(IFN-α),再與HRP標(biāo)記的IFN-α抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過*洗滌后加底物TMB顯色。TMBHRP酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的α干擾素(IFN-α)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計算樣品中α干擾素(IFN-α)濃度。

試劑盒組成

試劑盒組成

48孔配置

96孔配置

保存

說明書

1

1

 

封板膜

2片(48

2片(96

 

密封袋

1

1

 

酶標(biāo)包被板

1×48

1×96

2-8℃保存

標(biāo)準(zhǔn)品:45ng/L

0.5ml×1

0.5ml×1

2-8℃保存

標(biāo)準(zhǔn)品稀釋液

1.5ml×1

1.5ml×1

2-8℃保存

酶標(biāo)試劑

3 ml×1

6 ml×1

2-8℃保存

樣品稀釋液

3 ml×1

6 ml×1

2-8℃保存

顯色劑A

3 ml×1

6 ml×1

2-8℃保存

顯色劑B

3 ml×1

6 ml×1

2-8℃保存

終止液

3ml×1

6ml×1

2-8℃保存

濃縮洗滌液

20ml×20倍)×1

20ml×30倍)×1

2-8℃保存

Principle of the assay

The kit assay Rabbit IFN-α level in the sampleuse Purified Rabbit IFN-α antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN-α to wells, Combined IFN-α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN-α in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit

48determinations

96 determinations

Storage

User manual

1

1

 

Closure plate membrane

2

2

 

Sealed bags

1

1

 

Microelisa stripplate

1

1

2-8

Standard45ng/L

0.5ml×1 bottle

0.5ml×1 bottle

2-8

Standard diluent

1.5ml×1 bottle

1.5ml×1 bottle

2-8

HRP-Conjugate reagent

3ml×1 bottle

6ml×1 bottle

2-8

Sample diluent

3ml×1 bottle

6ml×1 bottle

2-8

Chromogen Solution A

3ml×1 bottle

6ml×1 bottle

2-8

Chromogen Solution B

3ml×1 bottle

6ml×1 bottle

2-8

Stop Solution

3ml×1 bottle

6ml×1 bottle

2-8

wash  solution

20ml×20 fold

×1bottle

20ml×30 fold

×1bottle

2-8

注:本產(chǎn)品僅供科研實驗使用,信息供參考,具體產(chǎn)品信息請來電或在線方式咨詢。
上海華壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd

 


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