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上海瑞齊生物科技有限公司

Anti-ERCC1 抗體說明書

時間:2011-12-1閱讀:511
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 Anti-ERCC1 抗體說明書

         

Quantity size :   100ul  (dilute with pH 7.4    0.01 M PBS or antibody diluent )

 

Background: Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF , which is also designated ERCC4 or ERCC11, is a protein that associates directly with the excision repair crosscomplementing 1 (ERCC1) factor . ERCC1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair . The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.

 

pecificity :   

        Anti-ERCC1 is a rabbit monoclonal antibody unconjugated                

         specific for ERCC1 of human, mouse and rat           

         use for western blotting,elisa,immunoprecipitation and immunohistochemistry

         Protein G affinity chromatography purification, purity :>95%

         Isotype:  IgG

         mol wt:  38 kDa

Anti-ERCC1 抗體說明書

Application :  

        Western blotting 1:100-500                

         Immunohistochemistry 1:100-500                 

         ELISA 1:500-1000                  

         Optimal working dilutions must  be determined by the end user.

 

Storage:  Store at -20 for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4

 

 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.

 

  

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