Anti-Smad3 抗體說明書
Catalog Number :HZ98123
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Smad proteins, the mammalian homologs of the Drosophila mothers against dpp （Mad） have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 （also designated Madr1 or JV4-1）, Smad5 and mammalian Smad8 （also designated Smad9 or MadH6） are effectors of BMP-2 and BMP-4 function while Smad2 （also designated Madr2 or JV18-1） and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 （also designated DPC4） has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
pecificity :
– Anti-Smad3 is a rabbit monoclonal antibody unconjugated
– specific for Smad3 of human, mouse, rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 54kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
